Introduction. The functional basis of protopectin complex can be represented as a network of regions that consist of homogalacturonan sequences and a base of rhamnogalacturonans-I, i.e. rhamnosyl-containing branching sites. Enzymatic isolation of these regions is possible only at a certain minimal native degree of polymerization. The research objective was to develop a system of criteria for assessing the potential applicability of the enzymatic transformation of plant protopectin complex. Study objects and methods. The research featured the polymerization degree of the homogalacturonan regions of the protopectin complex and produced a system of assessment criteria for the enzymatic fragmentation potential of the protopectin complex. The theoretical calculations were based on the values of the mass fractions of rhamnosyl and galacturonide residues in plant cell walls. The result was a new polymerization degree analytical function. Results and discussion. The ratio of the mass fractions of rhamnosyl and galacturonide residues in the water-insoluble plant tissue served as a dimensionless criterion of applicability. The rational condition for the dimensionless criterion of applicability was based on the fundamental constraint for homogalacturonan regions in the protopectin complex. It was expressed by a fundamental inequation. The rational area for determining the numerical values of the applicability criterion was presented as . The functional dependence was reduced to a two-dimensional criteria space as “width of rhamnosyl branches vs. the criterion of applicability”, where each pectin-containing raw material was given a single uniquely defined position. The boundary conditions for the criteria space were determined analytically. Conclusion. The new approach offers an assessment of the enzymatic fragmentation potential of the plant protopectin complex by homoenzyme preparations. The approach is in fact the second stage of the decision tree in the science-based technology for pectin and its products.
Introduction. The existing diversity of plant raw materials and products predetermine the prospects of studying their potential as sources of pectin substances. However all current classifications are either fragmented or inconsistent. Study objects and methods. Our theoretical ivestigation aimed to develop an adequate classification for all taxa of plant origin, as well as their tissues and derivatives as pectin-containing materials. We developed criteria for assessing transformation potential of the protopectin complex based on the mass fractions of biologically active non-uronide components, native water-soluble pectin, the protopectin complex, and pectin substances. Individual boundary conditions were based on individual pectin potential, protopectin fragmentation potential, and pectin isolation potential. Results and discussion. Based on the boundary conditions, we defined an universal criterion space that included a set of points M in the coordinates expressed by three main criteria. According to individual boundary conditions, the criterion space was divided, or zoned, into four domains corresponding to protopectin fragmentation potential. They were characterized by: 1) lack of pectin potential, 2) ineffective protopectin fragmentation, 3) ineffective isolation of fragmentation products, and 4) effective isolation. Finally, we developed a generalized algorithm to determine the location of points M[μ1, μ2 , μ3 ] in the zoned criterion space, characterizing the plant tissue. Conclusion. Our approach can be used to assess any plant tissue for its protopectin transformation potential, which determines the technological influence on its pectin potential. This approach is universal, i.e., applicable to both plant tissue and its derivatives.
The basic structural element of the cell walls of beet pulp is dietary fibers, therefore deep processing of this type of raw material is of considerable interest. One of the main physicochemical properties of dietary fibers used in food production is their water holding capacity. However, dietary fibers of beet pulp, as heterogeneous polymer components, are extremely labile to the conditions of their extraction. In this regard, it is relevant to identify technological factors and their rational sequence, in which the target properties will be expressed to the maximum extent. The authors developed an extensive algorithm for the sequential extraction of dietary fibers from beet pulp with selective processing by homofermental preparations, including various options for the preliminary preparation of raw materials. As a result, samples of beet fibers (mainly homogluacutronanes), corresponding to the key graph nodes of the developed algorithm, were identified. For each sample, the magnitude of water holding capacity was determined, as well as the Raman spectrum on an excitation wave of 785 nm with a spectrum scan duration of 3 s and 50 passes per spectrum. In the process of research, the key influence of the degree of saturation of the cell wall matrix with polyvalent metal ions was established. At the same time, in the presence of cations in the matrix, the number of fermentation stages of the same type and their duration play a key role in the formation of water-holding capacity. In the absence of cations, water holding capacity depends only on the nature of the polymer fermentation products. By the method of principal components, it was established that the water-holding capacity is closely related to the activity of oxygen atoms of glycosidic bonds between the links of the polymer chain, as well as the activity of hydroxyl groups not belonging to the carbohydrate ring. However, the main components used describe only 53.5% of the peak heights dispersion, which may indicate participation in the formation of peaks and any third-party factors, which indicates the necessity of further research.
Inclusion of minor components in the formulation of homogeneous food systems should inevitably result in some shift of redox equilibrium under conditions of interaction as "all with all." Chemical nature of components combined with properties of elements of medium chemical composition should determine direction and expression of such displacement. But due to the multicomponence of the medium itself and the small initial expression of redox properties for the added components, predicting the resulting properties of the formed food system becomes difficult. The aim of the work was to study the regularities of the influence of neutral minor components on the change of redox properties of homogeneous canned products from vegetable raw materials. Puree-shaped single-component natural canning products (puree) from fruit (apples, plum) and vegetable (pumpkin, carrots) raw materials are taken as research objects. Table salt (NaCl), sucrose and apple pectin were used as minor components. In the obtained canned product, active acidity (pH) and redox potential (Eh) were determined, and the reducing power index (RH) value was calculated. The studies showed that there was no statistically significant effect of NaCl on the redox properties of the food systems studied. There was a statistically significant effect of sucrose on the reducing power index of homogeneous canned puree from plums (in the range up to 18%) and carrots (in the range up to 10%). It has been found that inclusion of pectin in the composition of fruit and vegetable puree does not have a statistically significant effect on the reducing capacity of the product, except for stone fruit puree, for which there was statistically significant single step increase of RH within a weight fraction value of the minor component as 0.61-0.62%. In order to determine the mechanism of such a response, it is necessary to carry out an additional set of targeted studies.
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