Besides their protective role, polyamines also serve as signalling molecules. However, further studies are needed to elucidate the polyamine signalling pathways, especially to identify polyamine-regulated mechanisms and their connections with other regulatory molecules.Reduced height (Rht) genes in wheat are often used in breeding programs to increase harvest index. Some of these genes are encoding DELLA proteins playing role in gibberellic acid signalling. The aim of the present paper was to reveal how the mutations in Rht gene modify the polyamine-regulated processes in wheat. Wild type and two Rht mutant genotypes (Rht 1: semidwarf; Rht 3: dwarf mutants) were treated with polyamines. Polyamine treatments differently influenced the polyamine metabolism, the plant growth parameters and certain hormone levels (salicylic acid and abscisic acid) in these genotypes. The observed distinct metabolism of Rht 3 may more likely reflect more intensive polyamine exodus from putrescine to spermidine and spermine, and the catabolism of the higher polyamines. The lower root to shoot translocation of putrescine can contribute to the regulation of polyamine pool, which in turn may be responsible for the observed lack of growth inhibition in Rht 3 after spermidine and spermine treatments.Lower accumulation of salicylic acid and abscisic acid, plant hormones usually linked with growth inhibition, in leaves may also be responsible for the diminished negative effect of higher polyamines on the shoot growth parameters observed in Rht 3. These results provide an insight into the role of polyamines in plant growth regulation based on the investigation of gibberellininsensitive Rht mutants.

Highlights Putrescine pre-treatment increased cadmium toxicity in rice.  In contrast, putrescine synthesis inhibition alleviated cadmium stress.  The synthesis of higher polyamines and phytochelatins is antagonistically related.  Putrescine may decrease phytochelatin synthesis at enzymatic and gene expression levels. Although the metabolism of phytochelatins and higher polyamines are linked with each other, the direct relationship between them under heavy metal stress has not yet been clarified. Two approaches were used to reveal the influence of polyamine content on cadmium stress responses, particularly with regard to phytochelatin synthesis: putrescine pre-treatment of rice 2 plants followed by cadmium stress, and treatment with the putrescine synthesis inhibitor, 2-(difluoromethyl)ornithine combined with cadmium treatment. The results indicated that putrescine pre-treatment enhanced the adverse effect of cadmium, while the application of 2-(difluoromethyl)ornithine reduced it to a certain extent. These differences were associated with increased polyamine content, more intensive polyamine metabolism, but decreased thiol and phytochelatin contents. The gene expression level and enzyme activity of phytochelatin synthase also decreased in rice treated with putrescine prior to cadmium stress, compared to cadmium treatment alone. In contrast, the inhibition of putrescine synthesis during cadmium treatment resulted in higher gene expression level of phytochelatin synthase. The results suggest that polyamines may have a substantial influence on phytochelatin synthesis at several levels under cadmium stress in rice. Abstract