It has been reported that iron oxide nanoparticles have various biomedical applications, including cancer diagnosis and treatment. Iron oxide nanoparticles were known to exert cytotoxicity against MCF-7 breast cancer cell lines and in this present study, we have investigated for their apoptosis-inducing potential in the same cell line. The flow cytometry analysis of the MCF-7 breast cancer cell lines treated with functionalized iron oxide nanoparticles showed an increased percentage of cells in terms of viable, early, and late apoptosis. The cell cycle analysis of the MCF-7 cell lines treated with Syzygium aromaticum extract coated with polyvinylpyrrolidone (PVP) + iron oxide nanoparticles and PVP + iron oxide nanoparticles showed substantial accumulation of nanoparticles in the sub-G1 phase, confirming induction of apoptosis. The activities of caspase-3, caspase-8, and caspase-9 increased with increasing concentration of the nanoparticles indicating that activities of caspase can be activated by iron nanoparticles. Further, functionalized nanoparticles induced oxidative stress through reactive oxygen species (ROS) formation. Therefore, it is concluded that the functionalized iron nanoparticles induce apoptosis in MCF-7 breast cancer cell lines and further provides an opportunity to explore the iron nanoparticles for apoptosis in cancer treatment.
Magnetic iron oxide nanoparticles exhibit many interesting properties due to their surface chemistry and they can be exploited in biomedical and technological applications. The iron oxide nanoparticles (FeO NPs) using ferric chloride, ferrous chloride, PVP (Poly Vinyl Pyrolidone) and Syzygium aromaticum extract were successfully synthesized by wet-chemical co-precipitation method. The PVP coated FeO NPs and S. aromaticum loaded to PVP coated FeO NPs were systematically characterized by using expository tools. UV-Visible spectroscopy showed the maximum absorbance at 480 nm and 554 nm due to the excitation of surface plasmon vibrations in the FeO NPs formation. Fourier transform infrared (FT-IR) spectrum showed the presence of functional groups of in the form of various band positions and this study confirmed that phenols and primary amines were majorly present. DLS studies revealed narrow size of particles i.e., 399.8 nm and 190.1 nm. Particle size distribution was showed at 256 nm and 153 nm for these samples and zeta potential values were observed at-23.7 and-18.8 mV with greater stability. The X-ray diffraction (XRD) analysis is in good agreement with a high degree of crystallographic nature of particles and the average particle size was found to be 23 nm and 29 nm using Scherrer equation. FT-Raman spectrum showed three main characteristic peaks. The scope of this study was extended by examining the cytotoxicity of PVP coated FeO NPs and S. aromaticum loaded PVP coated FeO NPs on human breast (MCF-7) cancer cell lines. The results suggest that the S. aromaticum loaded FeO NPs acts as a potential anti-cancer agent and are a promising research field for lots of biomedical applications particularly for breast cancer studies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.