SynopsisAcrylarnide and methacrylamide derivntives of nucleic acid baws were prepared from the corresponding aminoethyl derivatives. Free-radical polymerization of these monomers in water or in organic solvents gave their polymers. From the studies of the polymer complex formation hy UV spectroscopy. it was found that extremely stable polymer complexes were formed between complementary polymers containing nucleic acid bases. 'For Part CXXX, see M. Miyata, K. Okanishi, and K .
Six chlorate-resistant (CR) cell lines were established from survivors after plating mutagenized and non-mutagenized cells of Kp20, a Paeonia haploid cell line, onto solid medium containing 30 mM KCIO3 and amino acids as the sole nitrogen source (AA-chlorate medium).Five millimolar N-ethyl-Nnitrosourea (ENU) was used as the mutagen. None of the 6 CR lines grew on medium containing nitrate as the sole nitrogen source (N03-medium). Of these CR lines, 5 completely lacked in vivo nitrate reductase (NR) activity and one showed low (about 11% of that of the wild type) NR activity.In all CR lines except for one, the percentage occupied by haploid cells increased to 83-95% compared with 73% in the wild type. This suggested that mutation may have occurred to advantage in the haploid cells.
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