In this study, we have extracted bioactive compounds from seabuckthorn (SBT) leaves. Total flavonoid content of SBT leaf extract estimated as rutin equivalent was found to be 116.98 AE 3.06 mg/g of extract. Further, we pre-treated aramid (Nomex IIIA) fabric with triethylene tetramine to increase the wickability of the fabric for better extract uptake. Surface etching on the amine treated fabric morphology was determined by scanning electron microscope analysis. Then, SBT leaf extract was coated using citric acid as cross-linking agent on to aramid fabric by pad-dry-cure method for improved wash durability. Universal attenuated total internal reflection Fourier transform infrared characterization of the SBT coated fabric showed distinct hydroxyl peak at 3305 cm À1 , carbonyl peak at 1728 cm À1 , and -C-O-C peak at 1083 cm À1 . There was no significant difference in the char produced by SBT coated fabric and control samples as estimated using flammability tester. Antimicrobial activity of the SBT coated fabric was assessed by both qualitative (agar diffusion method) and quantitative (percentage reduction test) methods using test organisms. An inhibition zone for Escherichia coli and Staphylococcus aureus was found to be 10.2 mm and 12.3 mm, respectively, by agar diffusion method. Quantitative assessment by percentage reduction test showed a reduction percentage of 96.00% and 93.00% for S. aureus and E. coli, respectively. Results suggested that SBT might be a valuable ingredient for the development of antimicrobial aramid textiles.
Flavonoid-rich fraction (FRF) from Seabuckthorn leaves extract was prepared by acid hydrolysis process. Total flavonoid content of Seabuckthorn leaves extract and FRF estimated as rutin equivalent was found to be 116.98±3.06 and 277.14 ± 6.78 mg/g of extract/FRF respectively. Its major constituents myrcetin, quercetin, kaempferol and isorhamnetin, were determined by reverse phase high performance liquid chromatography (RP-HPLC). Aramid (NomexIIIA) fabric was treated with triethylene tetramine to increase the wicking height of the fabric for better uptake of FRF. Then, FRF was coated using citric acid as cross linking agent on to aramid fabric by pad-dry-cure method for improved wash durability. FRF coated fabric was characterised using universal attenuated total internal reflection Fourier Transform Infrared spectroscopy. Effect of FRF coating on flammability property of coated fabric was estimated using flammability tester. There was no significant difference in the char length of the FRF coated fabric and control samples. Antimicrobial activity of the FRF coated fabric was assessed by both qualitative (agar diffusion method; AATCC 147-2001) and quantitative (percentage reduction test; (AATCC 100-2001) methods using test organisms. The zone of inhibition by agar diffusion method for E. coli and S. aureus was found to be 12.4 mm and 16.7 mm, respectively. Quantitative assessment by percentage reduction test showed a reduction percentage of 96.00 per cent and 93.00 per cent for S. aureus and E. coli, respectively. The results of the above study indicate FRF as a valuable ingredient for the development of antimicrobial textiles.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.