The effect of He-Ne laser radiation (lambda = 632.8 nm, I = 6.8 W/m2, irradiation time from 5 to 50 sec) on kinetics of spontaneous and Candida ablicans-stimulated chemiluminescence of mouse spleen cells was studied. It was found that laser radiation caused significant enhancement (180-250%) both of spontaneous chemiluminescence and Candida-induced chemiluminescence. The effective dose interval ranges from 100 to 300 J/m2, with a maximum at 200 J/m2. This finding shows that He-Ne laser irradiation can induce the respiratory burst (generation of reactive oxygen species having bactericidal activity) of phagocytic cells.
Chemiluminescence (CL) of splenocytes of A/Sn mice was recorded after irradiation of the cells with various individual laser an superluminous diode probes at wavelengths from 660 to 950 nm (pulse repetition rates varying from 4 to 5,000 Hz) and at various doses. Laser radiation was found to increase or suppress the spontaneous CL of splenocytes suspension, the amplitude and the sign of the effect depending on the cellular composition of the samples. Direct correlations between the effect of laser radiation (per cent in changes of CL when irradiated at 820 nm, 1.1 x 10(3) J/m2, 292 Hz) and per cent of plasmacytes (r = 0.743, P < 0.001), neutrophils (r = 0.650, P < 0.001) as well as myelocytes and metamyelocytes (r = 0.505, P < 0.01) were established. The correlation with per cent of lymphocytes (r = -0.590, P < 0.001) was found to be a reverse one. Dependence of the irradiation effects on dose, pulse repetition rate, and wavelength are presented.
Chemiluminescence (CL) of peripheral blood from the same donor was recorded after irradiation with various individual laser and superluminous diodes (660, 820, 880 and 950 nm, pulse repetition rates 16, 292 and 5000 Hz) during two periods of acute viral respiratory illness and in normal conditions of health. It was found that precise and statistically significant effects of laser radiation on CL (suppression of the CL) depend on wavelength, pulse repetition rate and dose and can be recorded only in the period of acute illness (i.e. at a certain immunological status of the organism) and there are practically no effects of laser radiation when the blood of a healthy donor is irradiated. The optimal irradiation parameters for suppression of free radical processes in human blood were as follows: dose range 10 3 -10 4 J/m2, pulse repetition rate 292 and 5000 Hz (16 Hz was ineffective). All wavelengths under study had the effect but l = 660 nm was found to be most effective (65% of CL was suppressed).
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