UPLC is a modern analytical technique which gives a new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, which enhance mainly in three areas: "speed, resolution and sensitivity. In twenty first centenary pharmaceutical industries are focusing for new analytical methods which are economy and shorten time for development of drugs. The separation and quantification of UPLC is done under very high pressure (up to 15000 psi). This review includes theories & principles of Chromatography along with the Comparison between HPLC and UPLC and also most recent applications of UPLC are also included.
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