Abstract. The effect of the inoculation and the adding of the cellulase enzyme on the fermentation of sugar-rich, direct cut grass were studied. The control silages were made by using AIV II solution and no additives. The silages were prepared from the first cut on a farm scale in the summer of 1985.Generally all four silages were organoleptically good. The results showed only minor differences in the fermentation between untreated and inoculated or enzyme silages. The quality of fermentation, however, was improved in inoculated and enzyme silages. The quality further improved by using the AIV II solution. This was supported by the changes in the pH, ammonia, buffering capacity, redox potential, temperature and finally by a better digestibility of organic matter.
A titration method has been developed to analyze acid mixtures, in which the pK values differed by 0.5 to 1 pH units. Instead of the use of equivalence points a buffer capacity curve is numerically derived from the titration curve resulting in a presentation similar to chromatograms and spectra. The consumption of the NaOH titrant is determined within pH intervals of 0.2-0.6 units around the pK values of the components. The buffer capacity measured is expressed as the consumption of NaOH over the pH interval. The amounts of compounds are found from these results using simple models of chemometrics. The method has been applied to a mixture of formic and acetic acids with concentrations of 0-3 mmol/20 ml with an error of 0-0.05 mmol. Furthermore a curve-fitting method based on a theoretical equation of buffer capacity can be applied with an error slightly larger than with the previous method. In this case, the pK values were also computed. For titrations beyond the pH range 4-10, the buffer capacity of water has to be subtracted from the titration results. The whole procedure requires including the computations about 3-6 min.
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