Cordyceps have been known for various functional and valuable human health components such as: cordycepin, adenosine, and hydroxyethyl adenosine which led to high demand for their usage. There were more than 680 species of Cordyceps in the world. However, Cordyceps sinensis and Cordyceps militaris were the two most studied species, by these results, created pressure to look for other species that have at least similar functions or even better than Cordyceps sinensis and Cordyceps militaris. With that objective, diversity understanding was the initial step implemented. 20 specimens of Cordyceps spp. were collected from Bidoup Nui Ba. Those samples were examined for morphology such as fertile head size, stroma length, host weight, and length with microscopic for the sharp Perithecia, Asci, and Ascospore. Then those specimens were extracted DNA with the CTAB method for the PCR cycle with ITS5/ITS4 primers. PCR products were sequenced to get data for building a phylogenetic tree. Based on the finding of morphology, there were three samples of Cordyceps sphecocephala, which were parasitic on Hymenoptera and 17 samples of Cordyceps nutans which were parasitic on Hemiptera. Molecular phylogeny analyses of ITS supported the morphology results by dividing 20 samples into two clades, similar to what was found by morphology with high bootstrap support.
Endoparasitic Cordyceps species infect pest insects and other arthropods all throughout the world. Ophiocordyceps nutans like specimens were found in the Lat and Dung K’no of the Lac Duong district during entomogenous fungi forays in Bidoup Nui Ba National Park. However, observations made over the last two years (2021 - 2022) in various forest locations across different altitudinal ranges revealed that O. nutans only occurs in two high-altitude forests, with elevations between 1409 and 1589 m asl with the temperature fluctuating from 20,3 and 25,7°C. Morphological comparisons and molecular phylogenetic analyses using D1 - D2 led us to conclude that the neotropical specimens represent Ophiocordyceps nutans (Pat.). In this study, the parasitic host of O.nutans of Hemiptera and host habitas is the bark and leaves of Lasianthus bidoupensis (Rubiaceae). Because Ophiocordyceps nutans parasitize and kill different species of stinkbugs that pose a hazard to silviculture and agriculture, making it a viable biocontrol agent.
Ophiocordyceps nutans is an entomoparasitic ascomycetes and has long been used extensively in traditional medicine in Asia because of its great aesculapian value. A field survey was undertaken at the Bidoup-Nui Ba National Park to evaluate the local community's environmental awareness. The results show that public knowledge about Cordyceps collection is significant. However, these fungus resources are expected to decline. The ecosystem for the fungus is changing negatively because the local community, which comprises ethnic minorities, is under considerable pressure due to its need for a sustainable livelihood, its low educational attainment, and hard life conditions The study focuses on environmental conditions that support in-situ and ex-situ conservation of this species, such as the temperature, humidity, and light, so that it can continuous to contribute to the sustainable management and enhancement of biodiversity in Bidoup-Nui Ba National Park.
Salmonella enterica, Vibrio parahaemolyticus, Escherichia coli and Listeria monocytogenes are human pathogenic bacteria which could be contaminated with many types of food, especially meat products. Testing of food for those pathogens has become routine practice all over the world. However, since the available traditional detection methods are time consuming and labor intensive, there is a need for rapid, sensitive, specific and cost effective detection technique. In this study, a rapid and sensitive single tube Multiplex PCR method for simultaneous detection of those four bacterial pathogens by amplification of target genes gyrB of V. parahaemolyticus, invA of S. enterica, eaeA of E.coli and hly of L. monocytogene was established. Concentration of bacterial cells from food samples for DNA extraction is another problem in testing of food pathogens by PCR techniques. Metal hydroxide immobilization was applied prior to DNA extraction and found to be effective step to overcome this problem.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.