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Objectives: The objective of the present study is to formulate an exfoliant using natural ingredients along with diatoms. Skin care products are important factor to improve confidence in individuals. Health of an individual is mainly represented by skin which is the major part of the body. Skin can be affected by several environmental factors such as ultraviolet rays, pollution, dust, and climatic changes that can intensify issues related to skin. Environmental causes can be prevented from skin damage by treating with topical application of synthetic or herbal cosmetics.Methods: In this preparation, diatoms are used, derived from marine phytoplankton which may contain a small quantity of muddy sludge, sand, and debris. Other natural ingredients are kiwi fruit, almond, and coffee seeds. Esthetic ingredients used in this preparation include pH modifiers, viscolysers, and surfactants. Among these ingredients, diatoms and coffee seeds attribute to the exfoliating activity of the preparation.Results: The prepared gel was evaluated for various parameters such as color, odor, consistency, pH, viscosity, spreadability, washability, grittiness, foamability, irritability, and extrudability. The results were found to be satisfactory.Conclusion: The present work was attempted to make a herbal exfoliant using suitable base to form a gel. Since diatoms are natural exfoliating agent, they are incorporated into the formulation which increases the efficiency of the product. Various parameters have been evaluated providing satisfactory result and improve the appearance of the skin without any side effects.

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The key role of a cryoprotectant is to minimize the chemical and physical stress which occurs during cooling, freezing and thawing of semen. The difference between the cryoprotectant (CPA) occurs in their permeability coefficient and the structural model of the cryogenic agent. The beneficial effect of dimethylacetamide (DMA) as a cryoprotectant especially for sperms had been observed in several studies. The aim of the study was to study the cryoprotective effect of DMA in freezing the Malabari buck semen compared to glycerol. Ten ejaculates were taken from fourMalabaribucks . After preliminary evaluation sample split technique was followed with Tris based extender containing glycerol (6.7 per cent) as cryoprotectant (control) and Trisextender containing DMA (3 per cent) as cryoprotectant (treatment group). The semen straws (0.25mL) after filling were subjected for equilibration and manual freezing. Sperm kinetics was studied using computer-aided sperm analyzer. Pre-freeze and post-thaw evaluation included sperm viability, sperm abnormality, hypo osmotic test, acrosome integrity test and DNA fragmentation. Results indicated that inclusion of 6.7 per cent glycerol had significantly higher (p<0.05) post-thaw values than DMA. From our study we conclude that 6.7 per cent glycerol was better than 3 per cent DMA in cryopreservation of Malabari buck semen.