In order to elucidate effects of 24 epibrassinolide (EB) on the efficiency of in vitro regeneration of highbush blueberry (Vaccinium corymbosum L., cv. Brigitta blue), we analyzed variability of seven biopro ductive parameters in regenerant plants cultured on nutrient media differing in hormonal composition. The additive action of EB, cytokinin (2iP), and auxin (IAA) was discovered for several parameters, such as repro duction coefficient, weight of regenerant plants, and the content of anthocyanin pigments. The greatest reproduction coefficients were noted in regenerant plants cultured on the medium containing 0.25 mg/L EB, 7.25 mg/L 2iP, and 1.00 mg/L IAA, as well as on the medium with 0.05 mg/L EB, 2.00 mg/L 2iP, and 0.50 mg/L IAA. It is suggested that low concentrations of 2iP and EB, applied in certain combinations, can be used for the industrial in vitro propagation of regenerant plants of highbush blueberry varieties.Abbreviations: 2iP-6 (γ,γ dimethylallylamino)purine; BSbrassinosteroids; EB-24 epibrassinolide; GA-genuine antho cyanins; LA-leucoanthocyanins; RC E -reproduction coeffi cient for explants; RC S -reproduction coefficient for shoots; TAP-total content of anthocyanin pigments; WPM-nutrient woody plant medium.
Приведены результаты сравнительного исследования в опытной культуре на выработанных торфяниках верхового типа в Смолевичском р-не Минской обл. и в расположенном на 250 км севернее Докшицком р-не Витебской обл. биохимического состава плодов сортов клюквы крупноплодной (Oxycoccus makrocarpus Ait. Pers.) -раннеспелого Ben Lear и позднеспелого Stevens. При внесении минерального удобрения Basacot Plus 6 и микробного препарата МаКлоР в 5-и 10 %-ной концентрациях, а также некорневых обработок Экогум-комплексом выявлены основные тенденции О б р а з е ц ц и т и р о в а н и я:
The article describes results of a comparative study of variability of 14 quantitative characteristics of biochemical composition of fruits in sort rows of 4 species of introduced varieties of Ericaceae (Oxycoccus macrocarpus, Vaccinium corymbosum) and Actinidiaceae (Actinidia arguta, Actinidia kolomikta) families, which has shown its lowest dependence on genotype in O. macrocarpus and the greatest dependence in A. arguta while the species studied have similarities in the level of genetic determinancy of a number of characteristics of fruits biochemical composition. It has been shown that the lowest stability in sort rows of both species of Ericaceae fam. is typical of parameters of accumulating anthocyanins proper and leucoanthocyanins while the greatest stability – that of ascorbic acid, which is characterized by high variability in both species of Actinidiaceae family. Most species of introduced plants studied have the lowest variability in sort rows in content of soluble sugars and total amount of bioflavonoids in fruits, while the greatest one – in sugar-acid index and tannins content. It has been found out that V. corymbosum and A. arguta have little dependence of flavonols content in fruits on genotype while V. corymbosum and A. kolomikta – that of pectin substances with a strong dependence of parameters of accumulating the latter in O. macrocarpus and A. arguta.
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