As the use of adhesive restorative materials has increased during the last several years, interest in adhesive materials that release fluoride has also grown. The purpose of this study was to measure fluoride release from several adhesive restorative materials and to evaluate its effect on dentin resistance to demineralization and on bacterial metabolism in a modified in vitro system. Standardized cavities (1.8 mm in diameter) were prepared in bovine teeth that had been ground to dentin. One cavity in each tooth was restored with one of the following restorative systems: (a) Single Bond and Z100; (b) Single Bond and Tetric Ceram; (c) Fuji Bond LC and Z100; (d) Fuji Bond LC and Tetric Ceram; (e) Fuji II LC; or (f) Fuji IX GP. The other cavity in each tooth was "restored" with wax as a control. For each restorative system, 12 specimens were evaluated for fluoride release during the first 24 hrs after restoration placement. Dentin adjacent to the restored sites was subjected to lactic acid challenge (pH 4.3) for 3 hrs, after which calcium release was measured. Another 12 specimens in each group were stored for 24 hrs in de-ionized water, and were exposed to an S. mutans suspension (1:1 THB/de-ionized water and 50 mM glucose, A660 = 0.2) for 6 hrs, followed by calcium release and pH measurement. Bulk specimens of each material were also made and stored in water. Fluoride released from Fuji Bond LC, Fuji IX GP, and Fuji II LC in bulk was significantly greater than from the other materials. In the restored dentin specimens, increased resistance to demineralization from a lactic acid challenge was directly related to fluoride release. The same effects were seen as a result of the S. mutans challenge. While fluoride release from restorative materials increased the resistance of dentin to demineralization in this system, the clinical relevance of the findings is not known.

The purpose of this investigation was to characterize the enamel composition of teeth affected with the hereditary enamel disorders known as amelogenesis imperfecta. Teeth from 10 individuals representing all 3 major AI types (hypocalcified, n = 3; hypomaturation, n = 3; hypoplastic, n = 4) and 10 normal teeth were studied. Half of each tooth was used for histological and biochemical studies. The enamel protein content was estimated by amino acid analysis. The enamel mineral content (volume %) was determined from the calcium and/or phosphorus content. Calcium was measured using atomic absorption and phosphorus was determined colorimetrically. The mean enamel mineral content was reduced for all hypomaturation and hypocalcified AI teeth while hypoplastic AI enamel varied from normal to reduced compared with normal enamel. The enamel protein content was increased in all but one AI case (7 cases were examined for protein) compared with the normal enamel. The mineral and protein content in AI enamel showed a significant inverse relationship (R = -0.939, P = 0.001). This study shows that all three of the major AI groups can have subtypes associated with substantial decreases in the enamel mineral content, although hypomineralization appears most severe in the hypomaturation and hypocalcified AI types. The decreased mineral content was associated with an increased protein content in AI enamel. These findings provide further evidence that altered enamel mineralization in AI teeth likely involves abnormal post-secretory processing of the enamel proteins.

Epidermolysis bullosa (EB) is a group of conditions characterized by basement membrane and cellular defects that result in skin fragility and variable extra-cutaneous involvement. The teeth can be severely affected with marked enamel malformations. The purpose of this study was to characterize the structure and composition of teeth from individuals representing the major EB groups (EB simplex, dystrophic EB and junctional EB). Teeth were examined from 28 individuals with EB and 10 healthy people unaffected by EB. Teeth from individuals with junctional EB had marked enamel hypoplasia with varying abnormalities in the enamel structure. Minor structural defects of enamel, including areas of surface pitting, were seen in the other EB types. Although there was a slight reduction (approximately 10%) in the enamel mineral content in several dystrophic EB and junctional EB teeth, the mean mineral content was similar for all EB enamel types and normal enamel. This study shows that while individuals with junctional EB have marked alteration of the enamel structure, the composition may be normal to only mildly altered. Laminin-5, the molecular defect in junctional EB, is associated primarily with alteration in the amount and/or structure of enamel while the mineralization process appears relatively intact. The marked enamel hypoplasia in this EB type suggests that laminin-5 plays an important role in the secretory phase of enamel development.

The purpose of this study was to determine the F levels in plasma and molar enamel from rat pups whose mothers had received various levels of F during pregnancy and/or lactation. Rats were started on water containing 0 (Group I), 50 (Group II), or 100 (Group III) ppm F at the beginning ofpregnancy or on the day of delivery. The mothers and pups were killed 13 days after delivery, and plasma F levels, milk Flevels, and pup molar enamel F levels were determined. (Ericsson, 1969;Ericsson and Ribelius, 1970;. Baseline values of F content in developing rat molar enamel have not been reported, nor has the effect of increasing the maternal F intake on plasma F levels and enamel F uptake in the pups been documented.The purpose of this study was to determine the F levels in plasma and molar enamel from rat pups whose mothers had received various levels of F during the pregnancy and the lactation periods, or the lactation period alone. Mother rats of an additional three litters were given water containing 50 ppm F. One-half of the pups in each litter were removed from the mother while actually nursing, and the other half were taken from the mother one to two hours before plasma was obtained from the pups.The F in each of the plasma samples from mothers (mean volume: 0.99 ml + 0.026) and pups (mean volume: 0.62 ml + 0.10), milk samples (mean volume: 0.80 ml + 0.180), and pooled enamel samples (mean weight: 13.91 mg ± 5.07) was concentrated by the rapid microdiffusion method of Taves (1968) as modified by Whitford and Reynolds (1979). Fluoride analyses were done using an ion-specific electrode. The percent recovery values were established using the known addition method (Taves, 1968). The differences in mean values were statistically evaluated using the Student t test (signifcant if p < 0.05).Because initial milk F concentrations were unexpectedly high, human milk samples were analyzed in the same series with subsequent rat milk samples. To test for possible contamination from fur and skin, de-ionized water was dropped on and collected from two animals and assayed for F. Results.At each of the fluoride intake levels, the data collected from animals exposed to fluoride both pre-natally and post-natally and from animals exposed only post-natally were not significantly different. Thus, the pre-natal-pluspost-natal and post-natal-only data were combined in each of the three F intake groups. Group I contained four mother rats and a mean of 10.0 + 1.4 pups per litter; Group II, five and 7.6 + 3.8; and Group III, six and 8.0 + 1.8.The percent recovery achieved by the analytical method was: 82% for mothers' plasma, 98% for milk, 67% for pups' plasma, and 75% for pups' enamel. The measurement error was + 2%.877

We conducted this study to measure maternal plasma, fetal plasma, and fetal enamel fluoride concentrations for four hours following an oral F dose to near-term pregnant guinea pigs. We placed female guinea pigs on de-ionized (Group I) or 3-ppm-F (Group II) drinking water prior to breeding and during gestation. On the 57th day of gestation, we administered a maternal dose of NaF solution (0.6 mg F/kg) by stomach tube. We collected samples of maternal plasma, fetal plasma, and fetal enamel at baseline, at 15 and 30 min, and at one, two, and four h after administration of the dose. We assayed samples for F using a modification of the micro-diffusion and ion-specific electrode method. Group I mean baseline F values were: maternal plasma, 0.016; fetal plasma, 0.002; and fetal enamel, 7.0 ppm. Group II mean values were: 0.055, 0.004, and 19.0 ppm. After the maternal fluoride dose, the mean maternal plasma [F] rose sharply for 30 to 60 min and declined to about 50% of peak values by four h. Fetal plasma [F] changed less in absolute values, but similarly to maternal changes relative to baseline. Fetal enamel mean [F] rose more in Group II than in Group I. Baseline F status had an important effect on F uptake in fetal enamel following an acute maternal fluoride dose.