The present work deals with the processes involved in the abiogenic polycondensation of nucleotides adsorbed on the clay mineral kaolinite under the action of ultraviolet (UV) radiation. The dependence of the yield of synthesis products on irradiation dose was studied. The maximum yield corresponds to a 6-h exposure. The newly synthesized substances were analyzed by ion-exchange chromatography. Some fractions were studied for the type of bonds they contained by venom phosphodiesterase and RNase T2 enzymatic hydrolysis. It was determined that some of the products synthesized by exposure of AMP adsorbed on the surface of clay particles to UV radiation may be looked upon as oligonucleotides in which some fragments have 2'-5'-bonded and others 3'-5'-bonded nucleotides.
It is well known now that wide-spread natural conditions of temperature and moisture fluctuations on the clay surface are the most favourable for low temperature reactions of polycondensation.It deals with the redistribution of absorbed molecules on the clay surface in each cycle.The authors conducted the investigation concerned with the processes of abiological synthesis of oligonucleotides from adenosine cyclic 3',5'-phosphate in the presence of clay minerals-kaolinite and montmorillonite in conditions of temperature (40-80oc) and moisture fluctuations.The influence of aliphatic diamines (ethylenediamine and propylenediamine) on the yield of reaction products was investigated. The condensation was enhanced by ethylenediamine to more extent than by propylenediamine.
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