Considered in this paper are the factors influencing sensitivity of analytical devices based on surface plasmon resonance phenomenon. The decrease in temperature influence on sensitivity of these devices can be reached using thermo stabilization of the whole device with flow cells for studied substances. Adduced here are some results concerning the influence of deposition geometry for nano-dimensional gold layer covering the surface of operating element on sensitivity of these devices.
The article presents a new developed method, which is able to detect the chicken infectious bronchitis virus (IBV) antigen in real time in various buffer solutions, using the surface plasmon resonance (PPR) nanobiosensor of the Plasmon-6 device. The PPR method is hypersensitive to changes in external factors, including the interaction of antigen (coronavirus) and specific antibodies. If the interaction does not happen, the resonance occurs at other angular parameters of the position of the sensitive PPR element relative to the laser radiation. Therefore, the PPR method is becoming a new effective rapid technique of viral pathogen detection, which is important for effective control over infectious diseases spreading. The possibility of IBK virus detection by the PPR sensor response, with preliminary immobilization of antigen or antibodies, is shown, involving the device "Plasmon- 6". The duration of the experiment is about 2 hours, which significantly saves research time compared to other methods (6-48 hours). The changes in the resonance angle in the range of 360-500 angle. sec when the IBC virus antigen binds to serum antibodies in water (distilled) were detected. The angular shift of the nanosensor resonance was determined when the IBC virus antigen bound to the serum antibodies in the PBS, which averaged 354 angular seconds. The possibilities of using the PPR method for express detection of the coronavirus infections pathogen in animal fluids in real time are demonstrated in article. Taking into account the significant social and economic negative consequences of the Coronaviridae virus family members and considering the current situation with the worldwide spread of COVID-19, the representative of the coronavirus family – the Infectious Bronchitis virus has been selected as a model.
Organic film deposition in vacuum is fast developing scientific and industrial domain. We developed installation for deposition of organic films equipped with optical spectrometer for measurements in situ. We are developing new dyes aimed for application in waveguide sensor, nonlinear optics and studying film organisation during deposition. Fluorinated azo-dyes and azomethine dyes were synthesized at University of Applied Sciences Wildau and at the Institute of Organic Chemistry, Kyiv. Compounds were evaporated at a pressure of 10-3 Pa using resistive heated crucible. Glass and glass covered with polytetrafluoroethylene (PTFE) film are used as substrates. The films were studied with Polytec and StellarNet spectrometers and an atomic force microscope. Optical spectra of the dye films revealed, that some compounds were decomposed during evaporation. Several kinds of dyes were evaporated and deposited without decomposition. Some deposited films formed H-aggregates and other types of aggregates. AFM images of dye films showed that their morphology depends on the chemical structure of the compounds and on the nature of the substrate on which the film was grown.
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