The duration of maternal immunity was determined from 112 lambs born from vaccinated ewes with the homologous PPR vaccine "Nigeria 75/1" at day 90 and day 120 of pregnancy. Serum samples were collected from lambs starting from day 15 to day 150 after birth and analyzed using the PPR specific competitive ELISA. At day 75 and day 90 after birth, 70% and 95% of these lambs respectively became negative. So it is recommended to vaccinate lambs against PPR in the interval from 75 to 90 days after birth.
The Virology Laboratory of the Central Laboratory of Animal Diseases in Ivory Coast at Bingerville received samples of wild and domestic avian species between February and December 2006. An RT-PCR technique was used to test for avian influenza (AI) and highly pathogenic AI subtype viruses. Among 2125 samples, 16 were type A positive; of which, 12 were later confirmed to be H5N1. Fifteen of these 16 type A positive samples were inoculated into the chorioallantoic cavity of 11-day-old embryonated hens' eggs for virus isolation. Eight produced virus with hemagglutination titres from 1/64 to 1/512. The 4/16 M-RT-PCR positive samples, which were H5N1 negative, were shown to be H7 subtype negative. The diagnostic efficiency of the laboratory for the surveillance of H5N1 in Ivory Coast was demonstrated. The positive cases of H5N1 were from a sparrowhawk (Accipter nisus); live market poultry and in free-range poultry, where the mortality rate was approximately 20% (2/10) and 96.7% (29/30) respectively. Currently, investigations into intensive poultry farms have proved negative for H5N1. No human cases have been reported this time.
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