TORCH is a novel time-of-flight detector that has been developed to provide charged-particle identification between 2 and 10 GeV/c momentum. TORCH combines arrival times from multiple Cherenkov photons produced within a 10 mm-thick quartz radiator plate, to achieve a 15 ps time-of-flight resolu-* Corresponding author. those using a commercial Planacon MCP-PMT, and single photon resolutions approaching 80 ps have been achieved. The photon counting efficiency was found to be in reasonable agreement with a GEANT4 Monte Carlo simulation of the detector. The small-scale demonstrator is a precursor to a full-scale TORCH module (with a radiator plate of 660 × 1250 × 10 mm 3 ), which is currently under construction.
We report on the implementation of a wide-field time-correlated single photon counting (TCSPC) method for fluorescence lifetime imaging (FLIM). It is based on a 40 mm diameter crossed delay line anode detector, where the readout is performed by three standard TCSPC boards. Excitation is performed by a picosecond diode laser with 50 MHz repetition rate. The photon arrival timing is obtained directly from the microchannel plates, with an instrumental response of ∼190 to 230 ps full width at half maximum depending on the position on the photocathode. The position of the photon event is obtained from the pulse propagation time along the two delay lines, one in x and one in y. One end of a delay line is fed into the “start” input of the corresponding TCSPC board, and the other end is delayed by 40 ns and fed into the “stop” input. The time between start and stop is directly converted into position, with a resolution of 200–250 μm. The data acquisition software builds up the distribution of the photons over their spatial coordinates, x and y, and their times after the excitation pulses, typically into 512 × 512 pixels and 1024 time channels per pixel. We apply the system to fluorescence lifetime imaging of cells labelled with Alexa 488 phalloidin in an epi-fluorescence microscope and discuss the application of our approach to other fluorescence microscopy methods.
We report on wide-field time-correlated single photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single-photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwatts with wide-field data collection. We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans.
K E Y W O R D Sfluorescence lifetime imaging (FLIM), lightsheet microscopy, microchannel plate (MCP), SPIM, time-correlated single photon counting (TCSPC)
Photek (U.K.) and the TORCH collaboration are undertaking a three year development program to produce a novel square MCP-PMT for single photon detection. The TORCH detector aims to provide particle identification in the 2-10 GeV/c momentum range, using a Time-of-Flight method based on Cherenkov light. It is a stand-alone R&D project with possible application in LHCb, and has been proposed for the LHCb Upgrade. The Microchannel Plate (MCP) detector will provide a single photon timing accuracy of 40 ps, and its development will include the following properties: (i) Long lifetime up to at least 5 C/cm 2 ; (ii) Multi-anode output with a spatial resolution of 6 mm and 0.4 mm respectively in the horizontal and vertical directions, incorporating a novel charge-sharing technique; (iii) Close packing on two opposing sides with an active area fill factor of 88% in the horizontal direction. Results from simulations modelling the MCP detector performance factoring in the pulse height variation from the detector, NINO threshold levels and potential charge sharing techniques that enhance the position resolution beyond the physical pitch of the pixel layout will be discussed. Also, a novel method of coupling the MCP-PMT output pads using Anisotropic Conductive Film (ACF) will be described. This minimises parasitic input capacitance by allowing very close proximity between the frontend electronics and the MCP detector.
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