Summary — The clonal seed orchard studied consisted of 60 clones phenotypically selected in Douglas fir stands planted in France during the first half of the twentieth century. The genotype of every clone was characterised using isozyme techniques. Seven enzyme systems were studied, 1 was monomorphic, and 9 polymorphic loci were analysed. Seven loci (from 5 enzyme systems) were sufficient for genetic identification of each clone. Rare alleles were noticed at 7 of the 9 polymorphic loci studied. Three rare alleles were used to assess the selfing rate of individual ramets, but 2 of them might have led to erroneous results because of their selective disadvantage. The individual selfing rates, assessed from the rare allele transmission by pollen, were low (2-5%
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