Membranes of Zymomonas mobilis ZM4 were separated by centrifugation on discontinuous density sorbitol gradient using a 2‐step purification procedure. Four bands of respective densities 1.17 (L1), 1.20 (L2), 1.22 (H1), and 1.32 (H2) were obtained. NADH oxidase activity was detected in L1 and L2 fractions, indicating that they were derived from cytoplasmic membrane. H1 and H2 fractions gave a positive Limulus polyphemus lysate test of outer membrane endotoxin. Proteins of 2 cytoplasmic membrane bands and of 2 outer membrane bands showed respectively similar patterns when separated by electrophoresis.
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