Objectives-To study the in vitro adhesion of polymorphonuclear leucocytes (PMNLs) to endothelial cells in patients with Behcet's disease (BD), and the humoral and cellular factors which may contribute to adhesion. Methods-A total of 118 patients with BD and 60 healthy controls were studied. In vitro adhesion of chromium-51 labelled normal neutrophils to human umbilical vascular endothelial cell (HUVEC) monolayers were studied in the presence of normal serum or serum obtained from patients with BD. Adhesion of neutrophils from patients with BD to HUVEC stimulated with tumour necrosis factor (TNF), interleukin-1 (IL-1), and lipopolysaccharide (LPS) and adhesion molecule (CD11a, CDl1b, CD18 and L-selectin) expression on the patient's neutrophils and lymphocytes were determined, and the serum concentration of IL-8 was measured. Results-Sera from patients with BD were found to enhance the adherence of normal PMNLs to HUVEC monolayers in vitro.Patients' sera induced an increase in surface expression of CDila and CD18 on normal neutrophils and intercellular adhesion molecule-i (ICAM-1) expression on HUVECs. The number of CDlla positive neutrophils was greater in the blood of patients with BD than in that of healthy controls (89.4% v 71%; p < 0-001).
These results suggest that the Turkish version of BDCAF may be useful for assessing the classic triad of BS (oro-genital ulceration and eye involvement), but more experience is needed for its other parts.
SUMMARY Adenosine deaminase activity was determined in paired samples of serum and synovial fluid taken from patients with rheumatoid arthritis (n=12), reactive arthritis (n=13), and osteoarthritis (n=7), and the value of this investigation in the diagnosis of synovial swellings was assessed. Increased activity was found in the synovial fluid taken from patients with rheumatoid disease and reactive arthritis, though values were less raised in the latter. Synovial fluid taken from patients with osteoarthritis did not show significantly raised adenosine deaminase activity as compared with that of normal controls (n=3).Key words: rheumatoid effusions.Adenosine deaminase (ADA) is one of the main enzymes of the purine metabolic pathway, catalysing the deamination of adenosine to inosine and deoxyadenosine to deoxyinosine. During inflammatory reactions the enzyme is released into the extracellular fluid, and in serous effusions of differing pathology the levels of ADA activity increase considerably. This increase has been shown to correlate with the number of nucleated cells, particularly T lymphocytes and macrophages, present in the effusions.i 2 It has also been shown that pleural effusions due to rheumatoid arthritis (RA)
We describe antigens of Plasmodium falciparum recognised by murine monoclonal antibodies which by immunofluorescence react with the rhoptry organelles of the extracellular merozoite stage. Immunoblotting shows that the antibodies recognise two major parasite antigens of Mr 82 and 65 kilodaltons (kDa). Immunoprecipitations from detergent extracts of [35S]-methionine-labelled parasites show that the 82-kDa and 65-kDa antigens are parasite proteins. Pulse-chase experiments on synchronous parasite cultures show that the 82-kDa protein is synthesised during early schizogony and is later processed into the 65-kDa antigen in segmenting schizonts. In Nonidet P-40, these antigens are non-covalently associated with two other proteins of 40 kDa and 42 kDa. The 40/42-kDa doublet is synthesised in parallel with the 82 kDa antigen and persists, apparently unchanged, till the end of the cell cycle.
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