der Plas, L. H. W. 1994. Occurrence of polygodia! in plant organs and tissue culture of Polygonum hydropiper. -Physiol. Plant. 92: 595-600.Shoots of Polygonum hydropiper L. (waterpepper), especially in the leaves and flowerheads, contain significant amounts of the sesquiterpenoid polygodial, a compound with a potential use a.s a natural pesticide. The poiygodia! content of the tepals is particularly high: up to 8.5% of the dry weight. Roots and seeds do not contain detectahle amounts of polygodial. Polygodial containing organs e.g. leaves and tepa!s, were found to contain cavities. Fourier transform infra-red (ITIR) microspectroseopy demonstrated that polygodial or its congeners arc found in these cavities hut not in other tissues or celis. Comparable cavities containing polygodial-li!ce compounds were absent in the closely related species Polygonum persicaria L. Callus cultures and cell suspensions as we!l as root-and shoot cultures were initiated from mature P. hvdropiper plants. Polygodia] could he detected only in shool cultures.Our results indicate tha( functioning plastids may be essential for polygodia! production and cavities for its acctimulation.
Shoots of Polygonum hydropiper L. (waterpepper), especially in the leaves and flower‐heads, contain significant amounts of the sesquiterpenoid polygodial, a compound with a potential use as a natural pesticide. The polygodial content of the tepals is particularly high: up to 8.5% of the dry weight. Roots and seeds do not contain detectable amounts of polygodial. Polygodial containing organs e.g. leaves and tepals, were found to contain cavities. Fourier transform infra‐red (FTIR) microspectroscopy demonstrated that polygodial or its congeners are found in these cavities but not in other tissues or cells. Comparable cavities containing polygodial‐like compounds were absent in the closely related species Polygonum persicaria L.
Callus cultures and cell suspensions as well as root‐ and shoot cultures were initiated from mature P. hydropiper plants. Polygodial could be detected only in shoot cultures. Our results indicate that functioning plastids may be essential for polygodial production and cavities for its accumulation.
A high‐pressure liquid chromatographic procedure for the determination of the antifeedant polygodial in Polygonum hydropiper leaves was developed. For leaves, the method consists of a selective extraction with hexane: EtOAc (8:2), pre‐purification of the evaporated extract over a solid phase C2 column, separation with a C18 RP‐HPLC column and detection/quantitation by means of UV at 233 nm. As internal standard α‐ionone is used. Advantages of the method are its selectivity and speed. Various aspects such as extraction efficiency, recovery, peak purity and reproducibility have been evaluated. The minimal detectable quantity is 1 ng.
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