No abstract

A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inactivate all mycoplasmas.

No abstract

Potassium iodide, potassium bromide, and potassium chloride materially slow down the bacteria killing rates of iodine, bromine, and chlorine, respectively, in distilled water. The germicidal activity of elemental iodine in water does not increase as the pH is increased from pH 5.8 (the mean value fovind in unbuffered water containing 2.0 ppni free iodine) to 8.0 as might be expected if the germicidal activity of iodine could be attributed to hypoiodous acid. Chlorine is considerably less effective as a disinfectant for sea water than for fresh water when judged by activity against the gram-positive test organism S. faecalis. While this can be attributed, in part, to interference by the neutral chloride salts present in sea water, the presence of an initial high chlorine demand and difficulties encountered in maintaining available chlorine residuals after this demand has apparently been satisfied make it impossible to conclude that this is the only factor involved.