The oil of Acer truncatum Bunge seed (ASO) is rich in ω-9 (53.93%) and ω-6 (30.7%) fatty acids (FAs), and characterized by 3-7% nervonic acid (NA, C24:1ω-9). Evidence suggests that...

Background/Aim: The clinical course of acute leukemia is complicated, and it is often necessary to combine or change treatment methods due to the rapid increase and spread of malignant cells. In this study, the potential antileukemia activities of prepared garlic essential oil (GEO) and some organosulfur compounds contained therein were examined. Materials and Methods: Garlic essential oil component identification by gas chromatography-mass spectrometry (GC-MS). MTT assay evaluated cytotoxicity of tested samples. Leukemia cell differentiation was determined by NBT assay. Apoptosis and related mechanisms were investigated by western blotting. Results: GC-MS analysis confirmed that the two most abundant constituents, diallyl disulfide (DADS) and diallyl trisulfide (DATriS), constituted 80% of the composition. GEO and DADS exhibited the best effects in terms of significant production of intracellular reactive oxygen species (ROS), induction apoptosis and potentiation differentiation of human promyelocytic leukemia cell line HL-60 cells. The GEO-mediated apoptosis was alleviated by the free radical scavenger N-acetyl-L-cysteine (NAC). Conclusion: The anti-leukemia activity of GEO and organosulfur compound DADS through the action of ROS elevation was herein confirmed.The World Health Organization Cancer Report estimated that the cancer burden will increase greatly over the next 20 years worldwide, with an increase in new cases of at least 60% (1). Lifestyle behaviors and environmental factors are the main causes linked to the majority of cancer cases. Therefore, avoiding harmful chemicals can reduce cancer-causing exposure and is the most important strategy for cancer prevention. According to the Taiwan Ministry of Health and Welfare, leukemia, a cancer of the blood cells, ranks 8 th in terms of cancer incidence in Taiwan; it is also the number one childhood cancer, accounting for more than 30% of all childhood cancers, and acute nonlymphocytic leukemia accounts for more than 5% of childhood leukemia cases in Taiwan (2). In the US, leukemia is the fifth highest in terms of cancer incidence, and killed more than 22,000 people in 2019 (3).It is known that leukemia has a complicated course of disease development, and treatment planning for leukemia often requires combination therapy or a series of different therapy approaches (4). Currently, chemotherapy is the most common treatment for acute leukemia, although it has many critical disadvantages, including high toxicity and low selectivity. Additionally, patients often develop drug resistance to chemotherapy agents, and a high relapse rate is another problem. Despite continued advancement in treatment, only a small percentage of patients survive for more than five years.In addition to undergoing treatment with prescription drugs, many cancer patients also take natural supplements prepared from plant extracts. Many plant extracts have been 6345