Measured ice crystal concentrations in natural clouds at modest supercooling (temperature ;.2108C) are often orders of magnitude greater than the number concentration of primary ice nucleating particles. Therefore, it has long been proposed that a secondary ice production process must exist that is able to rapidly enhance the number concentration of the ice population following initial primary ice nucleation events. Secondary ice production is important for the prediction of ice crystal concentration and the subsequent evolution of some types of clouds, but the physical basis of the process is not understood and the production rates are not well constrained. In November 2015 an international workshop was held to discuss the current state of the science and future work to constrain and improve our understanding of secondary ice production processes. Examples and recommendations for in situ observations, remote sensing, laboratory investigations, and modeling approaches are presented.

Weather extremes have widespread harmful impacts on ecosystems and human communities with more deaths and economic losses from flash floods than any other severe weather-related hazards. Flash floods attributed to storm runoff extremes are projected to become more frequent and damaging globally due to a warming climate and anthropogenic changes, but previous studies have not examined the response of these storm runoff extremes to naturally and anthropogenically driven changes in surface temperature and atmospheric moisture content. Here we show that storm runoff extremes increase in most regions at rates higher than suggested by Clausius-Clapeyron scaling, which are systematically close to or exceed those of precipitation extremes over most regions of the globe, accompanied by large spatial and decadal variability. These results suggest that current projected response of storm runoff extremes to climate and anthropogenic changes may be underestimated, posing large threats for ecosystem and community resilience under future warming conditions.

Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation of cellular therapeutics requires demonstration of comparability of lines derived from different individuals and in different facilities. This requires agreement on the critical quality attributes of such lines and the assays that should be used. Working from established recommendations and guidance from the International Stem Cell Banking Initiative for human embryonic stem cell banking, and concentrating on those issues more relevant to iPSCs, a series of consensus workshops has made initial recommendations on the minimum dataset required to consider an iPSC line of clinical grade, which are outlined in this report. Continued evolution of this field will likely lead to revision of these guidelines on a regular basis.

Abstract. Disparities between the measured concentrations of ice-nucleating particles (INPs) and in-cloud ice crystal number concentrations (ICNCs) have led to the hypothesis that mechanisms other than primary nucleation form ice in the atmosphere. Here, we model three of these secondary production mechanisms -rime splintering, frozen droplet shattering, and ice-ice collisional breakup -with a sixhydrometeor-class parcel model. We perform three sets of simulations to understand temporal evolution of ice hydrometeor number (N ice ), thermodynamic limitations, and the impact of parametric uncertainty when secondary production is active. Output is assessed in terms of the number of primarily nucleated ice crystals that must exist before secondary production initiates (N (lim) INP ) as well as the ICNC enhancement from secondary production and the timing of a 100-fold enhancement. N ice evolution can be understood in terms of collision-based nonlinearity and the "phasedness" of the process, i.e., whether it involves ice hydrometeors, liquid ones, or both. Ice-ice collisional breakup is the only process for which a meaningful N INP here suggest that, under appropriate thermodynamic conditions for secondary ice production, perturbations in cloud concentration nuclei concentrations are more influential in mixed-phase partitioning than those in INP concentrations.

Unique ATP-inhibitable K ؉ channels (K ATP ) in the kidney determine the rate of urinary K ؉ excretion and play an essential role in extracellular K ؉ balance. Here, we demonstrate that functionally similar low sulfonylurea affinity K ATP channels are formed by two heterologous molecules, products of Kir1.1a and cystic fibrosis transmembrane conductance regulator (CFTR) genes. Co-injection of CFTR and Kir1.1a cRNA into Xenopus oocytes lead to the expression of K ؉ selective channels that retained the high open probability behavior of Kir1.1a but acquired sulfonylurea sensitivity and ATP-dependent gating properties. Similar to the K ATP channels in the kidney but different from K ATP channels in excitable tissues, the Kir1.1a/CFTR channel was inhibited by glibenclamide with micromolar affinity. Since the expression of Kir1.1a and CFTR overlap at sites in the kidney where the low sulfonylurea affinity K ATP are expressed, our study offers evidence that these native K ATP channels are comprised of Kir1.1a and CFTR. The implication that Kir subunits can interact with ABC proteins beyond the subfamily of sulfonylurea receptors provides an intriguing explanation for functional diversity in K ATP channels.

Abstract. The Paris Agreement sets a long-term temperature goal to hold global warming to well below 2.0 ∘C and strives to limit it to 1.5 ∘C above preindustrial levels. Droughts with either intense severity or a long persistence could both lead to substantial impacts such as infrastructure failure and ecosystem vulnerability, and they are projected to occur more frequently and trigger intensified socioeconomic consequences with global warming. However, existing assessments targeting global droughts under 1.5 and 2.0 ∘C warming levels usually neglect the multifaceted nature of droughts and might underestimate potential risks. This study, within a bivariate framework, quantifies the change in global drought conditions and corresponding socioeconomic exposures for additional 1.5 and 2.0 ∘C warming trajectories. The drought characteristics are identified using the Standardized Precipitation Evapotranspiration Index (SPEI) combined with the run theory, with the climate scenarios projected by 13 Coupled Model Inter-comparison Project Phase 5 (CMIP5) global climate models (GCMs) under three representative concentration pathways (RCP 2.6, RCP4.5 and RCP8.5). The copula functions and the most likely realization are incorporated to model the joint distribution of drought severity and duration, and changes in the bivariate return period with global warming are evaluated. Finally, the drought exposures of populations and regional gross domestic product (GDP) under different shared socioeconomic pathways (SSPs) are investigated globally. The results show that within the bivariate framework, the historical 50-year droughts may double across 58 % of global landmasses in a 1.5 ∘C warmer world, while when the warming climbs up to 2.0 ∘C, an additional 9 % of world landmasses would be exposed to such catastrophic drought deteriorations. More than 75 (73) countries' populations (GDP) will be completely affected by increasing drought risks under the 1.5 ∘C warming, while an extra 0.5 ∘C warming will further lead to an additional 17 countries suffering from a nearly unbearable situation. Our results demonstrate that limiting global warming to 1.5 ∘C, compared with 2 ∘C warming, can perceptibly mitigate the drought impacts over major regions of the world.

Abstract. In situ measurements of Arctic clouds frequently show that ice crystal number concentrations (ICNCs) are much higher than the number of available ice-nucleating particles (INPs), suggesting that secondary ice production (SIP) may be active. Here we use a Lagrangian parcel model (LPM) and a large-eddy simulation (LES) to investigate the impact of three SIP mechanisms (rime splintering, break-up from ice–ice collisions and drop shattering) on a summer Arctic stratocumulus case observed during the Aerosol-Cloud Coupling And Climate Interactions in the Arctic (ACCACIA) campaign. Primary ice alone cannot explain the observed ICNCs, and drop shattering is ineffective in the examined conditions. Only the combination of both rime splintering (RS) and collisional break-up (BR) can explain the observed ICNCs, since both of these mechanisms are weak when activated alone. In contrast to RS, BR is currently not represented in large-scale models; however our results indicate that this may also be a critical ice-multiplication mechanism. In general, low sensitivity of the ICNCs to the assumed INP, to the cloud condensation nuclei (CCN) conditions and also to the choice of BR parameterization is found. Finally, we show that a simplified treatment of SIP, using a LPM constrained by a LES and/or observations, provides a realistic yet computationally efficient way to study SIP effects on clouds. This method can eventually serve as a way to parameterize SIP processes in large-scale models.

In‐cloud measurements of ice crystal number concentration can be orders of magnitude higher than the precloud ice nucleating particle number concentration. This disparity may be explained with secondary ice production processes. Several such processes have been proposed, but their relative importance and even the exact physics are not well known. In this work, a six‐hydrometeor‐class parcel model is developed to investigate the ice crystal number enhancement, both its bounds and its value for different cloud states, from rime splintering and breakup upon graupel‐graupel collision. The model also includes ice aggregation and droplet coalescence, ice hydrometeor nonsphericity, and a time delay formulation for hydrometeor growth. Conditions to maximize the breakup contribution, as well as the effects of nonsphericity and turbulence, are discussed. We find that the largest enhancement of ice crystal number occurs for “intermediate” conditions, characterized by moderate updrafts and activation and nucleation rates. In this case, vertical motion is strong enough, and new hydrometeor formation limited enough, to sustain supersaturation as hydrometeors grow to larger sizes. After these larger hydrometeors form at sufficient number concentrations, the ice crystal number can be enhanced by a factor of 104 in some cases relative to the number generated by primary ice nucleation alone. Excluding ice hydrometeor nonsphericity limits secondary production significantly, and the parcel updraft can modulate it by about an order of magnitude.