Biodiesel has become an attractive substitute for fossil fuels due to its advantages such as lower bulk, higher energy density, non‐toxicity, and biocompatibility. Herein, a green and facile route is performed to synthesize magnesium oxide nanoparticles (MgO NPs) using Ziziphus mauritiana leaves extract. After that, the resulting MgO NPs are applied as an active catalyst to produce biodiesel from the same plant's seed oil. The MgO NPs were characterized by various techniques including X‐ray diffraction spectroscopy (XRD), UV–Vis spectroscopy (UV–Vis), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Energy‐dispersive X‐ray spectroscopy (EDX). Besides, the activity of the “green catalyst” was tested for the transesterification reaction for triglyceride conversion into biodiesel. The catalyst loading was optimized, and stability analysis was performed. Furthermore, the catalyst was recycled for six times for the transesterification of Ziziphus seed oil. The fuel properties of produced biodiesel were studied, and all are within the ASTM and the EN biodiesel standard specifications.
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