The persistence and infectivity of SARS-CoV-2 in different postmortem COVID-19 specimens remain unclear despite numerous published studies. This information is essential to improve corpses management related to clinical biosafety and viral transmission in medical staff and the public community. We aim to understand SARS-CoV-2 persistence and infectivity in COVID-19 corpses. We conducted a systematic review according to Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) protocols. A systematic literature search was performed in PubMed, Science Direct Scopus, and Google Scholar databases using specific keywords. We critically reviewed the collected studies and selected the articles that met the criteria. We included 33 scientific papers that involved 491 COVID-19 corpses. The persistence rate and maximum postmortem interval (PMI) range of the SARS-CoV-2 findings were reported in the lungs (138/155, 89.0%; 4 months), followed by the vitreous humor (7/37, 18.9%; 3 months), nasopharynx/oropharynx (156/248, 62.9%; 41 days), abdominal organs (67/110, 60.9%; 17 days), skin (14/24, 58.3%; 17 days), brain (14/31, 45.2%; 17 days), bone marrow (2/2, 100%; 12 days), heart (31/69, 44.9%; 6 days), muscle tissues (9/83, 10.8%; 6 days), trachea (9/20, 45.0%; 5 days), and perioral tissues (21/24, 87.5%; 3.5 days). SARS-CoV-2 infectivity rates in viral culture studies were detected in the lungs (9/15, 60%), trachea (2/4, 50%), oropharynx (1/4, 25%), and perioral (1/4, 25%) at a maximum PMI range of 17 days. The SARS-CoV-2 persists in the human body months after death and should be infectious for weeks. This data should be helpful for postmortem COVID-19 management and viral transmission preventive strategy.
Background: Human papillomavirus type 16 (HPV16) is the most prevalent etiology of cervical cancer in Indonesian women. The L2 minor capsid protein has considerable potential as a broad-protective antigen target of the cervical cancer vaccine strategies, yet the data on L2 gene variation is still minimal. In this research, we determined the variations of the HPV16 L2 gene sequences in Indonesian cervical cancer specimens. Methods: We cross-sectionally observed 23 DNA isolates of HPV16 positive cervical cancer specimens stored in the laboratory of the
Background: Human papillomavirus type 16 (HPV16) is the most common etiological cause of cervical cancer in Indonesian women. HPV16 L2 genetic variation analysis is important for vaccine strategies of cervical cancer, yet the data in Indonesia is still limited. In this research, we determined the variation of the HPV16 L2 gene sequences in Indonesian cervical cancer specimens. Method: We cross-sectionally observed a total of 23 DNA isolates of HPV16 positive specimens stored in the laboratory of the Center for Diagnostic and Research on Infectious Diseases (PDRPI Lab) Faculty of Medicine, Universitas Andalas, Padang, Indonesia. The HPV16 L2 genes were amplified, sequenced, and followed by DNA alignment, single nucleotide polymorphisms (SNPs) analysis, and phylogenetic tree reconstruction. Results: As many as 35 SNPs were found, consist of 18 synonymous SNPs (sSNPs) and 17 non-synonymous SNPs (nsSNPs). Amino acid variations were mostly detected at S269P (100%) and L330F (43.48%) with no variation in the immuno-protective region near L2 N-terminus. A total of 5 HPV16 phylogenetic sub-lineages were found closely related to A1 (n=5), A2 (n=12), A3 (n=2), A4 (n=3), and C (n=1). Conclusion: The variation of HPV16 L2 gene sequences was mostly located on the central region of the L2 sequences and the cross-protective region near the L2 N-terminus was particularly conserved. This study should enhance the information about HPV16 L2 gene variation in Indonesia.
Kabupaten Pesisir Selatan merupakan salah satu Kabupaten yang memiliki 15 kecamatan dengan jumlah penduduk terbanyak di Kecamatan IV Jurai. Jumlah kematian per tahun di Pesisir Selatan berfluktuasi. Pada tahun 2015 jumlah kematian bayi meningkat dari 6 menjadi Sebanyak 42 jiwa (laki-laki 29 dan perempuan 13 jiwa) dari tahun sebelumnya, AKABA sebesar 47 jiwa dari 18 jiwa di tahun sebelumnya. Balita dengan gizi burut ditemui sebanyak 16 orang dan jumlah ibu hamil yang cukup banyak yaitu sekitar 273 ibu hamil. Berdasarkan studi pendahuluan yang dilakukan oleh tim pelaksana kegiatan di Puskesmas Lumpo Kecamatan IV Jurai Kabupaten Pesisir Selatan diketahui bahwa belum adanya fasilitas pemeriksaan USG pada kehamilan dan fasilitas prenatal yoga di Puskesmas Lumpo, selain itu belum adanya kegiatan yang sama yang dilaksanakan di SD Negeri 016 dan MTsN Nagari Lumpo yang termasuk wilayah kerja Puskesmas Lumpo. Ada beberapa pemeriksaan yang dapat dilakukan saat kehamilan sebagai upaya deteksi dini maupun pencegahan komplikasi dan gangguan pertumbuhan pada janin. Antara lain dengan memantau pertambahan berat badan selama hamil, melakukan pemeriksaan umum (tanda-tanda vital), pemeriksaan fisik yang fokus pada payudara, abdomen, genitalia dengan tindakan inspeksi, palpasi, auskultasi dan perkusi, pemeriksaan ultasonografi (USG) serta melakukan pemeriksaan penunjang berupa pemeriksaan laboratorium (pemeriksaan Haemoglobin). Hasil dari kegiatan ini adalah adanya peningkatan kualitas hidup setiap kelompok sasaran sesuai dengan tahapan kehidupan setelah diadakannya kegiatan ini.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.