Rhizome extracts of some members of the medicinal Zingiberales are widely used in dietary intake as well as in the traditional system of medicine. Curcumin, the chrome orange-yellow coloring compound present in turmeric rhizomes, has long been known to possess antioxidant property. Crude methanol extracts of the rhizomes of 11 species--Alpinia allughas, A. galanga, Curcuma amada, C. caesia, C. leucorrhiza, Hedychium coronarium, H. coccineum, H. flavescens, Kaempferia galanga, Zingiber cassumunar, and Z. officinale--were evaluated for their antioxidant properties using sulfur free radical reactivity with curcumin as a reference indicator. Sulfur free radicals (GS.) are generated by irradiating 15 mM glutathione (GSH) solution using a 5100 Ci cobalt-60 gamma irradiator. As reference indicator for the reactivity with sulfur free radicals, we used the depletion of pure curcumin sample in vitro as determined by a simple spectrophotometric method. The addition of the supernatant from crude rhizome extracts to the reaction mixture significantly decreased the depletion of curcumin, indicating that these crude extracts possessed antioxidant properties. The relative curcumin protection by different crude extracts against GS. showed that Z. cassumunar gave the highest degree of radioprotection. Our results reveal the potential medicinal use of rhizomes of medicinal Zingiberales as dietary agents.

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Rubber latex wastewater contains high level of inorganic anions such as nitrate and phosphate which are more prevalent as groundwater contaminant. The applicability of biofilm forming bacterial isolates to treat rubber latex wastewater was investigated. Microscopic analyses (CLSM and SEM) evaluated that the major component of biofilm other than bacterial cell is EPS matrix. EPS was found to contain higher proportion of lipids and polysaccharide which are required for attachment and absorption of nutrients. ATR-FTIR and 1 H NMR revealed the functional groups present in EPS. Rhodococcus rhodochrous CTI-14 showed robust biofilm followed by Cellulosimicrobium sp. CTB-10. Out of six bacterial consortia tested, consortium-D efficiently removed 95% NO − 3-N and 75% PO 3− 4. Taguchi method revealed the optimum parameters to remove the nutrients. The consortium-D optimally reduced nitrate and phosphate at pH 7 at 37°C. Consortium-D composed of Cellulosimicrobium sp. CTB-10, Aeromonas veronii OS-01, Lysinibacillus sphaericus RTA-01 and Rhodococcus rhodochrous CTI-14 was found to be the most efficient bacterial consortium to treat rubber latex wastewater.

The Zizania latifolia Griseb. Turcz. ex Stapf., a perennial wetland wild rice species get infected by a basidiomycetous fungus Ustilago esculenta Henn that produces gall. Here we report the morphogenesis behaviour of this plant during gall transformation. The identity of Z. latifolia and U. esculenta were confirmed by comparing microsatellite and Internal Transcribed Spacer region of nuclear ribosomal DNA sequence with the public database. U. esculenta infection transforms the Z. latifolia into two distinct morphotypes such as gall forming and non-gall forming. The non-gall bearing morphotype exhibited pinkish microporous lamellar discs (15-20 numbers) filled with mycelia in the internodal spaces. Whereas the gall bearing morphotype fuses 3-4 nodes into compact spindle shaped hypertrophic tissue measuring 2.5 cm diameter x 8-10 cm length tapering at the tip end where dark brown coloured teliospores are deposited in linear sori. In vitro culture of lamellar discs and teliospores in Potato Sucrose Agar medium initially develops white colony 2.6-4.0 cm diameter later turned into yellowish brown after 7 -10 days and subsequent sporulation within 8-12 days. Spherical sporangium of 28 µm diameter developed on long sporangiophore 7 µm diameter filled with large number of spores 1-1.5 µm in diameters. The unsuccessful sporulation of Ustilago even after infection failed to develop gall in the host plants behave normal life cycle and are not consumed. The spore along with hypertrophic soft tissue is consumed as vegetable in the oriental countries.