Highlights
The
adc1
disruption of
Synechocystis
sp. PCC 6803 did not inhibit cell growth, as evidently shown by its similar growth with wild type, as well as its intracellular pigments.
The
Δadc1
mutant has the highest capacity to produce PHB up to 36.1 %w/DCW under nitrogen and phosphorus-deprived medium containing acetate (BG
11
-N-P+A).
Abundant PHB granules stained by Nile-Red are markedly visualized in
Δadc1
mutant under BG
11
-N-P and BG
11
-N-P+A when compared to those of wild type.
Transcript levels of
phaA
and
phaB
are mainly responsible to PHB content whereas the changed proportion of
phaC
and
phaE
is noted when a certain
phaC
transcript level is decreased.
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