The phosphorus of glucose-6-phosphate residue in starch (P(G6P)) was estimated specifically by means o f glucose-6-phosphate dehydrogenase (E.C.1.1.1.49) af2er acid hydrolysis. Sixty to 70 "1'0 of total phosphorus in potato starch was found to be P(G6P). I t was shown that the rest of phosphorus (Px) was incorporated as glucose-2-phosphate andlor glucose-3-phosphate, since it was determined as glucose by periodate oxidation, borohydride reduction, acid hydrolysis and treatment with alkaline phosphatase. The P x was more labile to acid and heat than P(G6P) and decomposed yielding inorganic phosphate.The phosphooligosaccharide ( D T . 3.6), which was prepared by the limit hydrolysis of potato starch with glucoamylase of Rh. delemar (a-1,4-Glucan glucohydrolase E.C.3.2.1.3) was fractionated by gel-filtration through Sephadex G-25 into fractions with D > . 2 to 7. Each fraction contained a mole of phosphorus per mole of the saccharide. The P(G6P) and the Px were enriched in the low and the high D Y . jractions, respectively. (Zusammenfasstmg siehe Seite 342; Rhsumk d la page 343)
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