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Different conventional methods for synthesizing zinc oxide nano-particles (ZnONPs) significantly involves physical and chemical routes. But, often these synthesis processes are quite expensive and also involve the profuse use of many hazardous reagents. To overcome these problems, we have employed a convenient single step phytochemical synthesis of ZnONPs by using abundantly available bioresource, green cardamom. Aqueous extract of green cardamom served the purpose of reducing agent in the formation of ZnONPs, as the extract contains a high amount of flavonoids which hold great reducing properties. Various characterization analyses such as XRD, UV-vis, FTIR, SEM has been done to confirm the formation of ZnONPs and their size and shape. Antibacterial activity of the green ZnONPs was identified against Gram-positive as well as Gram-negative bacterial strains. Significant growth inhibition was found against all. The further anti-cancerous potential of synthesized nanoparticles was also studied on hepatocellular cell lines showing its potential to be a trustworthy anti-cancerous agent.

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Introduction and Aim:Emergence of different applications of metallic nanoparticles in various field leads to innovation of new synthetic strategies. Besides being non-toxic to mammalian cells, zinc oxide nanoparticles (ZnONPs) has gained paramount attention due to its excellent antibacterial potential. This study illustrates a comparative analysis of antibacterial and cytotoxic activity of both phytochemically synthesized and chemically synthesized commercially available ZnONPs.   Materials and Methods:As a source of reducing agent, leaf extract of Coriander sativum was employed in case of green synthesis of ZnONPs. Several techniques, such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light Scattering (DLS) and Field emission Scanning Electron Microscopy (FESEM) were performed to characterize both green synthesized and commercial ZnONPs.Antibacterial potential of both theZnONPs were investigated on Gram-positive and Gram-negative bacterial strains to draw a correlative outcome. Hepatocellular cell line was used to determine the cytotoxic activity of both ZnONPs.   Results:Both the nanoparticles showed antibacterial and cytotoxic activity with measurable degree of difference.   Conclusion: From these studies it can be concluded, the green synthesized nanoparticles showed greater antibacterial as well as cytotoxic activity in comparison to the commercial ZnONPs.