Abstract.We develop an algorithm to compute pCO 2 in the Scotian Shelf region (NW Atlantic) from satellite-based estimates of chlorophyll-a concentration, sea-surface temperature, and observed wind speed. This algorithm is based on a high-resolution time-series of pCO 2 observations from an autonomous mooring. There is a gradient in the air-sea CO 2 flux between the northeastern Cabot Strait region which acts as a net sink of CO 2 with an annual uptake of 0.50 to 1.00 mol C m −2 yr −1 , and the southwestern Gulf of Maine region which acts as a source ranging from −0.80 to −2.50 mol C m −2 yr −1 . There is a decline, or a negative trend, in the air-sea pCO 2 gradient of 23 µatm over the decade, which can be explained by a cooling of 1.3 • C over the same period. Regional conditions govern spatial, seasonal, and interannual variability on the Scotian Shelf, while multi-annual trends appear to be influenced by larger scale processes.
In this study, we used comparative metaproteomics to investigate the metabolic activity of microbial plankton inhabiting a seasonally hypoxic basin in the Northwest Atlantic Ocean (Bedford Basin). From winter to spring, we observed a seasonal increase in high-affinity membrane transport proteins involved in scavenging of organic substrates; Rhodobacterales transporters were strongly associated with the spring phytoplankton bloom, whereas SAR11 transporters were abundant in the underlying waters. A diverse array of transporters for organic compounds were similar to the SAR324 clade, revealing an active heterotrophic lifestyle in coastal waters. Proteins involved in methanol oxidation (from the OM43 clade) and carbon monoxide (from a wide variety of bacteria) were identified throughout Bedford Basin. Metabolic niche partitioning between the SUP05 and ARCTIC96BD-19 clades, which together comprise the Gamma-proteobacterial sulfur oxidizers group was apparent. ARCTIC96BD-19 proteins involved in the transport of organic compounds indicated that in productive coastal waters this lineage tends toward a heterotrophic metabolism. In contrast, the identification of sulfur oxidation proteins from SUP05 indicated the use of reduced sulfur as an energy source in hypoxic bottom water. We identified an abundance of Marine Group I Thaumarchaeota proteins in the hypoxic deep layer, including proteins for nitrification and carbon fixation. No transporters for organic compounds were detected among the thaumarchaeal proteins, suggesting a reliance on autotrophic carbon assimilation. In summary, our analyses revealed the spatiotemporal structure of numerous metabolic activities in the coastal ocean that are central to carbon, nitrogen and sulfur cycling in the sea.
Using large-scale field surveys across 12 estuaries in two provinces in Atlantic Canada, we analyzed changes in phytoplankton and benthic macroalgal communities as well as the canopy structure of eelgrass beds and quantified their carbon and nitrogen storage with increasing eutrophication. As eutrophication increased, phytoplankton biomass increased on average 1.8 times and phaeopigments doubled. Among macroalgae, the epiphytic Ulothrix speciosa increased 40 times in New Brunswick, and benthic Ulva lactuca 670 times in Prince Edward Island covering 61% of the bottom. Eelgrass showed a significant increase in leaf length and declines in shoot density and aboveground and belowground biomass, consistent with increased shading by opportunistic algae. As eelgrass biomass declined, so did the carbon storage capacity of the habitat. Nitrogen storage only declined in belowground eelgrass beds due to increasing tissue nitrogen content above ground with eutrophication. Despite province-and species-specific responses of primary producers to nutrient loading, principal component analysis revealed an overall shift from perennial eelgrass to opportunistic macroalgae and phytoplankton with eutrophication at the regional scale, indicating generalized eutrophication effects on primary producer assemblages.
We applied two numerical methods to in situ hyperspectral measurements of remote sensing reflectance Rrs to assess the feasibility of remote detection and monitoring of the toxic dinoflagellate, Karenia brevis, which has been shown to exhibit unique absorption properties. First, an existing quasi-analytical algorithm was used to invert remote sensing reflectance spectra, Rrs(lambda), to derive phytoplankton absorption spectra, a(phi)Rrs(lambda). Second, the fourth derivatives of the a(phi)Rrs(lambda) spectra were compared to the fourth derivative of a reference K. brevis absorption spectrum by means of a similarity index (SI) analysis. Comparison of reflectance-derived a(phi) with filter pad measured a(phi) found them to agree well (R2=0.891; average percentage difference, 22.8%). A strong correlation (R2=0.743) between surface cell concentration and the SI was observed, showing the potential utility of SI magnitude as an indicator of bloom strength. A sensitivity analysis conducted to investigate the effects of varying levels of cell concentrations and colored dissolved organic matter (CDOM) on the efficacy of the quasi-analytical algorithm and SI found that a(phi)Rrs(lambda) could not be derived for very low cell concentrations and that, although it is possible to derive a(phi)Rrs(lambda) in the presence of high CDOM concentrations, CDOM levels influence the a(phi)Rrs(lambda) amplitude and shape. Results suggest that detection and mapping of K. brevis blooms based on hyperspectral measurements of Rrs are feasible.
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