Gray wolves (Canis lupus) were extirpated from the northern Rocky Mountains (NRM) of the United States by the 1930s. Dispersing wolves from Canada naturally recolonized Montana and first denned there in 1986. In 1995 and 1996, the United States Fish and Wildlife Service reintroduced 66 wolves into central Idaho and Yellowstone National Park. By 2008, there were ≥1,655 wolves in ≥217 packs, including 95 breeding pairs in the NRM. From 1993–2008, we captured and radio‐collared 1,681 wolves and documented 297 radio‐collared wolves dispersing as lone individuals. We monitored dispersing wolves to determine their pack characteristics (i.e., pack size and surrounding pack density) before and after dispersal, their reproductive success, and eventual fate. We calculated summary statistics for characteristics of wolf dispersal (i.e., straight‐line distance, age, time of year, sex ratio, reproduction, and survival), and we tested these characteristics for differences between sexes and age groups. Approximately, 10% of the known wolf population dispersed annually. The sex ratio of dispersals favored males (169 M, 128 F), but fewer dispersed males reproduced (28%, n = 47) than females (42%, n = 54). Fifty‐nine percent of all dispersers of known age were adults (n = 156), 37% were yearlings (n = 99), and 4% were pups (n = 10). Mean age at dispersal for males (32.8 months) was not significantly different (P = 0.88) than for females (32.1 months). Yellowstone National Park had a significant positive effect on dispersal rate. Pack density in a wolf's natal population had a negative effect on dispersal rate when the entire NRM population was considered. The mean NRM pack size (6.9) from 1993 to 2008 was smaller than the mean size of packs (10.0) from which wolves dispersed during that time period (P < 0.001); however, pack size was not in our most supported model. Dispersals occurred throughout the year but generally increased in the fall and peaked in January. The mean duration of all dispersals was 5.5 months. Radio‐collared wolves dispersed between Montana, Idaho, and Wyoming to other adjacent states, and between the United States and Canada throughout the study. Mean straight‐line distance between starting and ending points for dispersing males (98.1 km) was not significantly different than females (87.7 km; P = 0.11). Ten wolves (3.4%) dispersed distances >300 km. On average, dispersal distance decreased later in the study (P = 0.006). Sex, survival rate in the natal population, start date, dispersal distance, and direction were not significant predictors of dispersal rate or successful dispersal. Wolves that formed new packs were >11 times more likely to reproduce than those that joined packs and surrounding pack density had a negative effect on successful dispersal. Dispersal behavior seems to be innate in sexually mature wolves and thereby assures that genetic diversity will remain high and help conserve the NRM wolf population. © 2017 The Wildlife Society.

The last sentence in the Abstract section should read, "We recommend collecting samples <7 days old and estimate that a sampling interval of four to seven days in summer conditions (i.e., extreme heat and exposure to UV light) will achieve desired sample sizes for mark-recapture analysis while also maximizing efficiency". The only change made to the sentence was '>' changed to '<'. The publisher apologizes for the error. Reference Woodruff SP, Johnson TR, Waits LP (2015) Evaluating the interaction of faecal pellet deposition rates and DNA degradation rates to optimize sampling design for DNA-based mark-recapture analysis of Sonoran pronghorn. Molecular Ecology Resources, 15, 843-854.

Population abundance estimates are important for management but can be challenging to determine in low-density, wide-ranging, and endangered species, such as Sonoran pronghorn (Antilocapra americana sonoriensis). The Sonoran pronghorn population has been increasing; however, population estimates are currently derived from a biennial aerial count that does not provide survival or recruitment estimates. We identified individuals through noninvasively collected fecal DNA and used robust-design capture-recapture to estimate abundance and survival for Sonoran pronghorn in the United States from 2013 to 2014. In 2014 we generated separate population estimates for pronghorn gathered near 13 different artificial water holes and for pronghorn not near water holes. The population using artificial water holes had 116 (95% CI 102-131) and 121 individuals (95% CI 112-132) in 2013 and 2014, respectively. For all locations, we estimated there were 144 individuals (95% CI 132-157). Adults had higher annual survival probabilities (0.83, 95% CI 0.69-0.92) than fawns (0.41, 95% CI 0.21-0.65). Our use of targeted noninvasive genetic sampling and capture-recapture with Sonoran pronghorn fecal DNA was an effective method for monitoring a large proportion of the population. Our results provided the first survival estimates for this population in over 2 decades and precise estimates of the population using artificial water holes. Our method could be used for targeted sampling of broadly distributed species in other systems, such as in African savanna ecosystems, where many species congregate at watering sites.

Understanding the spatial use of wolves and how that might relate to prey species may help predict areas with increased likelihood of wolf–prey interactions, areas where wolves may have a higher impact on prey populations, or areas of wolf–livestock conflict. After reintroduction into Yellowstone National Park in 1995, wolves Canis lupus expanded south and recolonized areas in and around Grand Teton National Park in the southern Yellowstone ecosystem in Wyoming, USA. Elk Cervus elaphus in this area are supplementally fed at three feedgrounds artificially increasing elk density. We tracked radio-collared and uncollared wolves annually in winter (December–March) from 2000 to 2008 to investigate kill sites. Our objective was to investigate potential differences in habitat variables (e.g., canopy cover, elevation) between kill sites (n = 295) and available (random; n = 2,360) locations and investigate whether factors influencing winter wolf kill sites differed in a natural setting (i.e., native winter range) vs. an artificial setting (i.e., near or on feedgrounds). Wolf kills occurred at sites with lower elevation, canopy cover, and terrain roughness compared with random locations. Wolf kills were also slightly farther from packed surfaces (i.e., roads or groomed snowmobile trails) and elk feedgrounds, although still in areas of higher intensity of use by elk compared with random locations. Kill sites on native winter range were considerably more rough (odds ratio = 4.47) than those on feedgrounds. Our results suggest wolves hunt where the likelihood of encountering prey is high, although in areas where prey distribution is more sparse (i.e., native winter range), wolves may need to rely on rougher terrain for successful hunts. The relationship between areas of high prey use and increased wolf activity has important implications for both wildlife managers and livestock producers. In the future, managers will continue to face the issue of having high concentrations of ungulates, either wild or domestic, and the obvious attraction this has for wolves.

The Sonoran pronghorn (Antilocapra americana sonoriensis) is a subspecies of pronghorn found exclusively in the Sonoran Desert of Arizona (USA) and Mexico. Sonoran pronghorn persist at low densities and are geographically isolated from other pronghorn populations. Numbers have declined in recent decades, but the population has rebounded from a low of fewer than 50 animals in 2003 to an estimated 159 individuals in 2012; however, little is known about population demographics beyond abundance estimates. We developed a species identification test that uses mitochondrial DNA (mtDNA) species-specific primers to distinguish between sympatric Sonoran pronghorn and mule deer (Odocoileus hemionus) using DNA extracted from fecal pellets. We accurately identified each species in 100% of the blood and tissue reference samples. We also evaluate the rate of DNA degradation in pronghorn fecal samples ranging from 1 day to 124 days old and document that mtDNA species identification success rates were 100% through day 14. Success rates dropped to 95% by day 21, 50% on day 60, and 10% by day 124. This new test will be a valuable tool for documenting the presence of Sonoran pronghorn across their current range and can also be used for other pronghorn populations. Ó 2014 The Wildlife Society.