SUMMARYCultures of brain cells taken from mice at I to 3 days after peripheral infection with Chikungunya virus, showed loss of contact inhibition and morphological alterations which suggested that these cells may have been transformed. In cultures infected in vitro with a low dose of about I0 ~ LDs0Jml of culture medium, areas of epithelial-like cells and hypertrophied cells were dominant, cultures were destroyed after infection with a high dose of about IO 6 LDs0/ml of culture medium. Cultures not destroyed by virus were studied for up to 4 months. The supernatant fluids of these cultures were titrated regularly in an adult mouse system in which the amounts of virus recorded varied from I0 ~'7 to 10 6.o LDs0/ml.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.