A patient with myelofibrosis, who has produced many red blood cell autoantibodies, is described. Although the patient is phenotypically R1R1 (CDe/CDe), eluates made from his red blood cells have consistently contained what appeared to be anti-E, and more recently another antibody that appeared to be anti-c. In in vitro experiments we have shown that the "anti-E" and "anti-c" can be totally adsorbed by E-negative and c-negative red blood cells, respectively. We conclude that the two antibodies have quite different specificities from those indicated by simple antibody identification studies, and that both are more closely related to the anti-Hr series of antibodies than to anti-E or anti-c.
Tests in which 11 examples of anti-U were used in titration studies
against the red blood cells of 9 obligate Uu heterozygotes, from 4 unrelated families,
and random Negro and Caucasian donors (many of whom were of the presumptive
UU genotype) have failed to demonstrate any dosage of the U antigen.
A solid-phase erythrocyte adherence assay has been developed for the serological detection of reagin antibodies in syphilis. CaptureS (Immucor, Inc., Norcross, Ga.) is a nontreponemal, qualitative screening test for the detection of immunoglobulin G (IgG) and IgM antilipid antibodies in serum or plasma samples from blood donors. The CaptureS assay utilizes a modified Venereal Disease Research Laboratory antigen bound to microtitration wells and anti-IgG-plus anti-IgM-coated indicator erythrocytes as the detection system. The CaptureS assay was evaluated at six separate sites on 10,942 specimens. For patient samples of clinically diagnosed syphilis categories (n ؍ 366), the CaptureS assay yielded a sensitivity of 80.7% versus 80.3% for the rapid plasma reagin (RPR) card test (Becton Dickinson Microbiology Systems, Cockeysville, Md.). In comparative experiments on patient and donor samples (n ؍ 10,222), the CaptureS assay demonstrated a sensitivity of 94% compared to 91.2% for the RPR card test. The CaptureS and RPR card tests produced essentially equivalent specificities of 99.2% and 99.3%, respectively, for this sample population. For five test sites, the CaptureS and RPR card test demonstrated a 98.3% agreement (10,085 of 10,264) of test results. These evaluations indicate that the CaptureS compares favorably to the RPR card test in assay sensitivity and specificity, with the added benefits of ease of use, accommodation of high-volume testing, and potential for automation.
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