Abstract. Arrufitasari PN, Sutjahjo SH, Wirnas D. 2022. Performance of tomato M7 mutant lines and their similarities to the parents based on SSR markers. Biodiversitas 23: 1239-1245. Tomato cultivation in the lowlands can be an alternative to increase tomato production so that high-yielding and lowland adaptive varieties are required. This study was aiming at determining the performance of M7 mutant lines and their similarities to the parents based on SSR markers. The experiments were conducted at KP Leuwikopo (250 m asl) and Plant Molecular Biology Laboratory, IPB University, Indonesia. The results showed that the G4 line had the highest productivity among the tested genotypes (36.72 ton ha-1) and significantly differed from all check varieties. The highest number of fruit per plant was 51.67 in the G7 line. On the other hand, G2, G3, G7, G8, and G10 lines had the lowest percentage weight and the number of fruits cracking and were not significantly different from the check varieties (Tora and Mawar). A total of 7 SSR primers detected 87 alleles with a range of 7-15 alleles per locus. The similarity between mutant lines and their parents ranged from 44-69%. The mutant most closely resembled the parent was the G3 line, while the most different based on molecular markers was the G9 line.
Genetic variability is critical in order to create candidates for new superior varieties. This research objective was to evaluate the performance and variability of agronomical traits on the mungbean SSD F4 V422H/129 population. The research was conducted at IPB University, Bogor, from March to June 2021. The SSD F4 VR422H/129 population (378 genotypes with single plant as a representative of one genotype) and five check varieties were evaluated. The treatments were arranged in augmented design for RCBD in three replications. For agronomical traits, every F4 plant and 10 sample plants for each check variety were observed. Statistical analysis includes mean, frequency-distribution, heritability, genetic variability, correlation. The days to flowering, days to harvesting, generative periods, plant height on F4 V422H/129 were not significantly different from Vima 5, while total pod number, total pod weight, total seed weight, pod length were significantly higher. The agronomical traits were affected by genetic factors (heritability 0.71-0.99%), except for days to harvest. Based on the high genetic variability (CGV 0.62-0.64%), it was possible to select of total pod weight, total seed weight, or pod length in the next generation V422H/129. The total seed weight had a significant positive correlation with total pod weight and pod length.
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