This article aims to examine the effect of acceptance factors on the quality of e-learning systems among HEI students in Malaysia to meet the needs of teaching and learning in line with developed countries. This study is beneficial to the world of Education, especially at the level of Higher Education Institutions that make the e-learning system one of the main mediums in the teaching and learning system. This study uses a quantitative approach through a cross-sectional survey method. The findings of the study show the perception of ease of use (β=0.342, p<.05; technical support (β=0.260, p<.05); lecturer characteristics (β=0.203, p<.05) and the perception of usefulness (β=0.181, p<.05) has a positive effect on the quality of the e-learning system. This proves that the acceptance factors influence the quality of the e-learning system among HEI students in Malaysia. The advancement and use of this technology is important to enable the country to keep pace with the country-developed countries and can produce highly knowledgeable human capital to contribute to progress in the country's education and socio-economic systems.