This study was designed to explore the role of lncRNA LINC00616 in the regulation of periodontitis. Cellular functions were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays. The content of reactive oxygen species, Fe 2+ , glutathione, and malondialdehyde were measured to determine ferroptosis in Porphyromonas gingivalis lipopolysaccharide (LPS-PG) treated periodontal ligament stem cells (PDLSCs), as well as expression of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11, and acyl-CoA synthetase long-chain family member 4 proteins mRNA and miRNA levels were measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Western blot analysis was performed to assess protein expression. Targeting relationships were predicted using StarBase and TargetScan and verified by a dual luciferase reporter assay. The lncRNA LINC00616 was upregulated in periodontitis ligament tissues of patients with periodontitis and in PDLSCs treated with LPS-PG. Inhibition of LINC00616 promoted cell viability and suppressed ferroptosis of PDLSCs. miR-370 was verified to be a target of LINC00616, and suppressed miR-370 reversed the effects of LINC00616 knockdown on cell viability and ferroptosis in PDLSCs. Additionally, miR-370 targeting the transferrin receptor protein and upregulated transferrin receptor (TFRC) abolished the effects of overexpressed miR-370 on cell viability and ferroptosis of PDLSCs. LINC00616 acted as a competitive endogenous RNA (ceRNA) to promote ferroptosis of PDLSCs via the miR-370/TFRC axis. Therefore, LINC00616 knockdown may be a promising therapeutic strategy for periodontitis.
Background: In recent years, annual incidences of adenoid hypertrophy (AH), a highly common tissue lesion in children, have increased. Currently, research on AH has focused on its obstruction of nasal cavity function, and little has been written on its influence on the upper airway's bone structure. For this reason, our present study seeks to determine the influence of AH on both the morphological development characteristics of the upper airway and the craniofacial features in children, with the goal being to offer more choices for diagnosing and treating the condition in the future.
The current experiment was carried out to observe and analyze the effects of three orthodontic appliances combined with minocycline on periodontitis and inflammatory factors of gingival crevicular fluid. the patients included in this study were 180 patients with dental crowding treated in our hospital. They were divided into three groups, namely the invisible orthodontic appliance group (n=60), self-ligation orthodontic appliance group (n=60), traditional ligation bracket group (n=60), all of which were applied with minocycline therapy. The therapeutic effects of the three groups were observed. after three months of treatment, the periodontal index of each group showed an upward trend (p<0.05). At six months to one year, the periodontal index of the self-ligation orthodontic appliance group and conventional ligation bracket group was higher than that of the invisible orthodontic appliance group, p<0.05. After treatment, the expression of IL-1β and TNF-α increased for the three groups, p<0.05; the self-ligation orthodontic appliance group and the traditional ligation bracket group were the same as the invisible orthodontic appliance group in at 24 months, p>0.05. in the early stage of orthodontic treatment, the invisible orthodontic appliance can keep the oral cavity in a clean and hygienic state. However, after 18 months of orthodontic treatment, all three orthodontic treatments did not have a significant impact on oral hygiene.
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