The efficacy of a single cell protein (SCP) methanotroph (Methylococcus capsulatus, Bath) bacteria meal (FeedKind®, Calysta, Menlo Park, CA, United States), in Pacific white shrimp (Penaeus vannamei) diets was studied to determine growth performance, survival rate and disease resistance against Vibrio parahaemolyticus causing Acute Hepatopancreatic Necrosis Disease (AHPND). The growth trial was assigned in a completely randomized design (CRD) with four treatments and 5 replicates of each, T1: a fishmeal-based control containing 15% fish meal and 3 diets with graded levels of methanotroph bacteria meal, namely T2: 5% methanotroph bacteria meal, T3: 10% methanotroph bacteria meal, and T4: 15% methanotroph bacteria meal. Shrimp were fed ad libitum for 6 weeks on trial diets to assess growth. Subsequent to the growth trial, three replicates of the same groups were exposed to V. parahaemolyticus by a single bath challenge and held for a further 15 days on the same diets as the growth study to assess survival and resistance. No significant differences (p > 0.05) in survival or in growth performance, including final weight, weight gain, specific growth rate, feed consumption or feed conversion ratio of white shrimp fed feeds containing methanotroph bacteria meal or control diets for 6 weeks. Immune markers such as hemocyte counts, phenoloxidase, superoxide dismutase and lysozyme activity were similar across all groups after the 6-week feeding trial. In a V. parahaemolyticus challenge, methanotroph bacteria meal in the diet significantly promoted the survival rate, and the reduction of Vibrio sp. in the hepatopancreas of white shrimp. Hemocyte count and phenoloxidase activity showed no significant differences (p > 0.05) between diet treatment groups, but hemolymph protein was significantly higher (p < 0.05) in shrimp fed diets containing 15% methanotroph bacteria meal after challenge. The Vibrio colony counts from hepatopancreas in the treatment groups were all significantly lower than the control (p < 0.05). The findings show that methanotroph bacteria meal can entirely replace fishmeal in white shrimp diets and the 15% inclusion of methanotroph bacteria meal in shrimp diet shows no adverse effects on growth performance, feed utilization and survival rate. In addition, shrimp fed methanotroph bacteria meal diets exhibited improved survival rates to an AHPND challenge.

Background and Aim: Data about dynamics of serum biochemical parameters and negative energy balance (NEB) related conditions differ among geographical areas or farm types. It should be cautious about applying those data to justify control and prevention strategies of NEB problems. Therefore, dynamics of blood biochemical parameters related to NEB condition of peripartum dairy cows raised in either smallholder (SH) or semi-commercial (SC) farms were studied. Materials and Methods: Thirty-two healthy pregnant dry cows were selected from five dairy farms in Western part of Thailand, including 15 and 17 cows from three SH and two SC farms, respectively. Blood samples were collected at 2 weeks before the expected calving date, and 1, 2, 4, and 8 weeks postpartum to determine concentrations of glucose, non-esterified fatty acid (NEFA), and β-hydroxybutyrate (BHBA). Body condition scores (BCSs) and milk yields were also recorded. Results: Dairy cows in both farm types suffered from NEB by elevation of serum NEFA and BHBA, and loss of BCS postpartum. Degrees of elevation in serum NEFA and BHBA were different between farm types. The SC cows showed more BCS loss postpartum, but lower serum NEFA. In the SH cows, even with less BCS loss, the cows showed high serum NEFA concentrations. Conclusion: Dairy cows in our study entered NEB condition even with low milk yields. Moreover, elevation of serum NEFA and BHBA postpartum was higher in our studied cows as compared with other studies in high producing cows of commercial dairy farms in temperate areas.

To consider diet optimization for the growth and health of fish under intensive aquaculture systems, with a focus on the farming of Nile tilapia and red tilapia in Thailand, we conducted an experiment based on a completely randomized design (CRD), with three treatments and four replicates. Three diets, supplemented with different trace minerals, were applied to selected groups of fish: (a) a control diet, without organic trace minerals supplementation; (b) a T1 diet of chromium-L-methionine at 500 ppb, in combination with a zinc amino acid complex at 60 parts per million (ppm); and (c) a T2 diet of chromium-L-methionine at 500 ppb in combination with selenomethionine at 300 ppb. Red tilapia with an initial mean weight of 190 ± 12 g/fish were randomly distributed into cages of 2 × 2 × 2.5 m in a freshwater pond (12 cages in total), with 34 fish per cage and a density of 17 fish/m3. During the 8 week feeding trial, the fish were fed 3–4% of their body weight twice a day. The fish were weighed, then blood samples were collected to study their immune responses. The intestines were collected, measured, and analyzed at the end of the feeding trial. The results showed that the red tilapia that were fed with diets of chromium-L-methionine in combination with a zinc amino acid complex in the T1 treatment had significantly (p < 0.05) higher final weights, weight gains, average daily gains (ADGs), and better feed conversion ratios (FCRs), compared with fish that were fed with the control diet without organic trace minerals and with fish that were fed with the T2 diet (p < 0.05). The midgut and hindgut villus heights of the group fed with chromium-L-methionine in combination with a zinc amino acid complex in the T1 treatment were significantly higher than those of the other groups (p < 0.05). The levels of the antioxidative enzyme superoxide dismutase (SOD) and lysozyme activity were not significantly different from those of fish that were fed with the control diet (p > 0.05), whereas the glutathione level tended to increase (p < 0.1) in fish that were fed with chromium-L-methionine in combination with selenomethionine in the T2 treatment. Therefore, we concluded that chromium-L-methionine in combination with a zinc amino acid complex or selenomethionine clearly enhanced red tilapia’s growth performance and feed utilization through the promotion of antioxidative enzyme activity and immune response.

Since Asian sea bass is one of the economically most important fish, aquaculture conditions are constantly optimized. Evidence from feeding studies combined with the current understanding of the importance of zinc for growth and immune defense suggest that zinc supplementation may be a possible approach to optimize aquacultures of Asian sea bass. To investigate the effects of zinc deficiency and zinc supplementation, cells from Asian sea bass were incubated in culture medium with different zinc contents. The expression of genes, important for zinc homeostasis, redox metabolism, and growth hormones was analyzed using RT-PCR. Zinc deficiency induced the expression of certain zinc transporters (ZIP14, ZIP10, ZIP6, ZIP4, ZnT4, ZnT9) as well as of SOD1, IGF I and IGF II, while expression of ZnT1 and metallothionein (MT) was reduced. Zinc supplementation decreased the expression of ZIP10, while expression of ZnT1 and MT were elevated. No differences in the effects of zinc supplementation with zinc sulfate compared to supplementation with zinc amino acid complexes were observed. Thus, extracellular zinc conditions may govern the cellular zinc homeostasis, the redox metabolism and growth hormone expression in cells from Asian sea bass as reported for other fish species. Our data indicate that supplementing aquacultures with zinc may be recommended to avoid detriments of zinc deficiency.

This study aimed to  investigate the effect of green tea waste, fibrolytic enzymes (xylanase and cellulase) and phytase  supplementation in layer  diets on   performance and egg quality. The experiment was assigned in a 2×3 factorial arrangement by completely randomized design.  Factor A consisted of two types  of diets: a) a basal diet without any green tea waste   and b) a basal diet with 1% of green tea waste.  Factor B consisted of three  levels of enzymes: a) level 0X, no enzyme supplementation; b) level 1X, a combination of fibrolytic enzymes,  24,000 FAXU xylanase and 100 ECU cellulase, and 1,000 FTU phytase; and c) level 2X, combination of fibrolytic enzymes,  48,000 FAXU xylanase and 200 ECU cellulase, and 2,000  FTU phytase.  One hundred  and ninety- two   Roman  Brown hens, aged 52 weeks, were randomly distributed to  six   groups and four replications. Layer hens were fed commercial diets with green tea waste and fibrolytic enzymes added on top as assigned in the treatment. The results showed that  green tea waste   significantly  increased (p<0.05) egg production, egg mass, feed conversion,  and Haugh unit.   In addition, green tea waste  tended  to affect (p=0.07) TBAs value.  Furthermore, the enzyme level significantly decreased  (p<0.05) feed intake and TBAs value.  In conclusion, the supplementation of 1% of green tea waste and 2X enzymes can  improve feed  conversion   and  the antioxidative  property of  eggs.