Effects of Zinc Status on Expression of Zinc Transporters, Redox-Related Enzymes and Insulin-like Growth Factor in Asian Sea Bass Cells

Abstract

Since Asian sea bass is one of the economically most important fish, aquaculture conditions are constantly optimized. Evidence from feeding studies combined with the current understanding of the importance of zinc for growth and immune defense suggest that zinc supplementation may be a possible approach to optimize aquacultures of Asian sea bass. To investigate the effects of zinc deficiency and zinc supplementation, cells from Asian sea bass were incubated in culture medium with different zinc contents. The expression of genes, important for zinc homeostasis, redox metabolism, and growth hormones was analyzed using RT-PCR. Zinc deficiency induced the expression of certain zinc transporters (ZIP14, ZIP10, ZIP6, ZIP4, ZnT4, ZnT9) as well as of SOD1, IGF I and IGF II, while expression of ZnT1 and metallothionein (MT) was reduced. Zinc supplementation decreased the expression of ZIP10, while expression of ZnT1 and MT were elevated. No differences in the effects of zinc supplementation with zinc sulfate compared to supplementation with zinc amino acid complexes were observed. Thus, extracellular zinc conditions may govern the cellular zinc homeostasis, the redox metabolism and growth hormone expression in cells from Asian sea bass as reported for other fish species. Our data indicate that supplementing aquacultures with zinc may be recommended to avoid detriments of zinc deficiency.

ZNT1 Regulates the Proliferation, Migration and Invasion of HaCaT Cells Infected with HPV Through the PI3K/Akt Pathway

“…[ 13 ] Homeostasis of Zn 2+ is maintained by the zinc-transporting proteins from the solute carrier family 39 and solute carrier family 30. [ 14 ] Solute carrier family 30 member 1 (SLC30A1, also known as ZNT1) is a member of the ZnT family and plays an important role in epidermodysplasia verruciformis, another disease caused by HPV infection. [ 15 ] In recent years, high expression of ZNT1 has been reported in the epidermis of CA patients infected with HPV, and its expression level has been positively correlated with HPV risk grade.…”

The Effect of Chronic Immobilization Stress on the Content of Micro- And Macroelements in the Pancreas and Blood Serum of Rats and Their Newborn Offspring

“…Changes in the level of micro-and macroelements in the tissue of the pancreas and blood serum are not absolutely identical, which indicates the presence of organ specificity regarding the balance of macro-and microelements, which means the relative informativeness of blood serum indicators for determining the state of metabolic processes in individual organs, in particular in the pancreas gland Probably, the negative effect of chronic stress on the composition of macro-and microelements is realized through an increase in the blood serum levels of precursors and metabolites of catecholamines, which contribute to a decrease in their content due to the stimulation of the secretion of such hormones as parathormone and cortisol, the absorption of Ca in the intestines is inhibited and its content in the body decreases. increases excretion of Mg-magnesiumuria, which regulates the transmembrane transfer of calcium and sodium ions, independently participates in many metabolic reactions related to the production, accumulation, transfer, and utilization of energy, free radicals, and their oxidation products, transmembrane transfer of calcium and sodium ions, affects the function of the nervous system and the coordination of all processes, including the formation of adequate adaptive and adaptive reactions and stress resistance [12,15,20,21] There are data that during stress in the organism, there are phase changes in the content of Zn in the homogenate of the PZ tissue: the accumulation of Zn levels in the PZ cells with an increase in corticosterone and corticotropin in the blood passes into a phase of a decrease in Zn concentration with a decrease in the levels of these hormones [22]. In a healthy pancreas, Zn is concentrated in granules and is contained in β-cells, and is necessary for the normal production and storage of insulin, Zn exhibits an insulin-mimetic and antidiabetic effect [23], is a coenzyme of many enzymes (carbonic anhydrase and lactate dehydrogenase), as well as superoxide dismutase, which protects the body from the products of peroxidation of lipids, the damaging effect of which is also realized during stress.…”