The present study aimed to evaluate in vitro anti-adherence abilities of the probiotic potential of three selected lactic acid bacteria (LAB) strains isolated from fermented broken rice with two foodborne pathogenic bacteria to Caco-2 cells. Lactobacillus plantarum Ban Pra Doke 110 was the most adherent strain with an adherence of 17.66% at a concentration of added bacterial cells of 2.0 × 10 8 cfu/mL (multiplicities of infection 100). In the exclusion, competition and displacement tests, Lact. plantarum PD 110 showed the most anti-adherence abilities against Salmonella Typhimurium American Type Culture Collection 13311 and Listeria monocytogenes ScottA to Caco-2 cells. Moreover, the presence of its spent culture supernatant exhibited the highest decrease in the percentage numbers of the adherent pathogenic bacteria used in all tests. The effectiveness of each LAB strain was tested to adhere to Caco-2 cells or to prevent the adherence of the test pathogenic bacteria varied widely depending on the genus and species, source of origin, and assay condition, as well as adherent-promoting factors. PRACTICAL APPLICATIONSFoodborne pathogen infection to the human intestinal epithelial surface cells is a critical step for the survival and cause foodborne illness. The anti-adherent assessment of novel probiotic is of importance for the inhibition of pathogen infections. Lactobacillus plantarum PD 110 isolated from fermented broken rice could be one of these good candidates. The demonstrated in vitro anti-adherent activity of Lact. plantarum PD 110 to Caco-2 cells recommends its possible application as a probiotic candidates in order to inhibit the adherence of Salmonella Typhimurium and Listeria monocytogenes to the human gastrointestinal tract. Therefore, this strain can be considered as a novel potential starter culture to improve the quality and safety of functional foods. bs_bs_banner Journal of Food Safety ISSN 1745-4565 IN VITRO ANTI-ADHERENT ASSESSMENT S. SRIBUATHONG, J. SAENGPRAKAI and S. TREVANICH