Mandible is the hardest and most durable bone of the skull exhibiting a high degree of sexual dimorphism. Especially ramus of mandible is subjected to greater stress than any other bone of the skull because of the process of mastication. This study has been performed to establish the osteometric standards for practical use in forensic context over Indian population using mandibular ramus. The sample consists of 116 mandibles of Northern Indian population (M:F; 92:24, mean age 37.4 years), collected from the Department of Forensic Medicine, IMS, BHU, Varanasi. Osteometric informations about five metric parameters (coronoid height, projective height, condylar height, and maximum breadth and minimum breadth of ramus) were taken with sliding calipers. These parameters were subjected to different discriminant function analysis using SPSS 16.0. All parameters showed significant sexual dimorphism (p < 0.001 in all cases) with an overall accuracy of 80.2%, and coronoid height was the single best parameter providing an accuracy of 74.1%.

No abstract

Determination of sex from fragmentary crania is a critical problem in forensic anthropology. Osteometric analysis of mastoid can serve forensic anthropologists better in sex identification by virtue of the noticeable dimorphic characteristics. The present study aims to develop population-specific, sex-differentiating anthropometric standards for the mastoid process of North Indian skulls. Eight parameters of the mastoid region were measured on 138 adult crania (M/F 104:34, 22-65 years) and analyzed using SPSS 16.0. All parameters showed significant sexual dimorphism (p < 0.000). In stepwise analysis, asterion-mastoidale and mastoid breadth have provided an accuracy of 87%. Receiver operating characteristic curves were obtained for each variable to observe their overall performance in sex determination. Posterior end of incisura mastoidea-depression of suprameatal triangle was found to be the best variable with maximum area under curve and highest predictive accuracy (82.6%).

Sex identification is an initial crucial objective in the revelation of the complete identity of the human skeleton as it also renders significantly clearer guidance towards age and stature estimation. Population specific standards are of great practical relevance in the present era of increasing population intermixing. Size differences and robusticity are the two well-elaborated pillars holding most of the dimorphic burdens of the skull. This study is designed to explore dimorphic characteristics of the craniofacial region to establish anthropometric standards for contemporary North Indian populations, which have not been available so far. One hundred and twelve adult crania of known age (23-65 years) and sex (M:F; 82:30) were collected in the Department of Forensic Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. Ten standard metric parameters of craniofacial region were measured and subjected to stepwise and direct discriminant function analysis employing SPSS 16.00. Bizygomatic breadth emerged as the single best parameter in stepwise analysis, providing an average accuracy of 85.5%.

Background: Although well studied the association between chronic typhoid carrier state and carcinoma of the gallbladder (CaGB) remains unproven. Methodology: The study was performed at a tertiary care medical center in North India and involved 52 patients with CaGB, 223 patients with benign gallbladder diseases, 508 healthy individuals and, 424 corpses. For the detection of Salmonella enterica serovar Typhi, hepatobiliary specimens were subjected to DNA extraction for specific nested-PCR amplification of the S. Typhi flagellin gene. Anti-Vi S. Typhi antibodies were detected in serum samples from patients by indirect haemagglutination. Results: Thirty five of the 52 (67.3%) CaGB patients were PCR-positive for the S. Typhi flagellin gene; significantly higher than for patients with benign gallbladder diseases (95/223, 42.6%; p<0.01) and corpses (35/424, 8.2%; p<0.001). The numbers of individuals that had significant anti-Vi antibody titres (≥160) in their serum were 20/52 (38.5%) for CaGB patients, 31/223 (13.9%) for patients with benign gallbladder diseases, and 47/508 (9.2%) for healthy individuals. Conclusions: Specific nested-PCR amplification of the S. Typhi flagellin gene in hepato-biliary specimens was more sensitive for detection of S. Typhi carriage than anti-Vi antibody titres in serum. The results demonstrate an association between typhoid carriage and gallbladder diseases, both CaGB and benign. S. Typhi specific immunosuppression is also suggested in patients with gallbladder diseases.

Determination of sex of unknown skeleton remains is the most important step in the identification process. Racial and regional differences in the populations create and maintain specificity in their dimorphic characteristics. Moreover, considering continued secular changes in the population structure, constant revision of osteometric standards becomes mandatory. In an effort to establish osteometric standards for the femur of contemporary North Indian populations, 122 adult femora of known sex (M: 94; F: 28) were collected in the Department of Forensic Medicine, IMS, BHU, Varanasi. Eight standard parameters were measured and analyzed by discriminant function analysis using SPSS 16. The accuracy of sex prediction ranged from 70.5% to 83.6% with single variables. In stepwise analysis, epicondylar breadth, proximal breadth, and antero-posterior diameter of the lateral condyle were found to be the most discriminating variables providing an accuracy of 90.2%. The results clearly indicate the importance of the ends of femur in the determination of sex.

No abstract

Wistar rats (male) were divided into 3 groups - group A (GA) served as control, group B (GB) were daily administered chlorpyrifos (Anu Products Ltd., India) orally at a dose of 5 mg/kg b wt. and animals in group C (GC) received daily an oral administration of chlorpyrifos at a dose of 10 mg/kg b wt. Rats were sacrificed on 1st, 2nd, 4th, 6th and 8th week after initiation of the experiment. Kidneys were extirpated and fixed in aqueous Bouin's solution. The tissues thus fixed were routinely processed for histological studies. The present study showed that the histopathological changes were caused in kidney of rats by chlorpyrifos administration. The changes noticed were mainly the shrinkage of glomerulus at initial stage of treatment, the tubular dilation, the glomerular hypercellularity, hypertrophy of tubular epithelium, degeneration of glomerulus and renal tubules, deposition of eosin-positive substances in the glomerulus and renal tubules and infiltration of leucocytes. A decrease in the body weight gain was observed in chlorpyrifos-treated rats. However, variable intensities of these changes were noticed depending upon the doses and duration of the treatment.