The contribution of microRNAs to lymphoma biology is not fully understood. In particular, it remains untested whether microRNA dysregulation could contribute to the emergence of the aggressive subset of B-cell lymphomas that coexpress MYC and BCL2. Here, we identify microRNA-124 (miR-124) as a negative regulator of MYC and BCL2 expression in B-cell lymphomas. Concordantly, stable or transient ectopic expression of miR-124 suppressed cell proliferation and survival, whereas genetic inhibition of this miRNA enhanced the fitness of these tumors. Mechanistically, the activities of miR-124 towards MYC and BCL2 intersect with both oncogenic and tumor-suppressive pathways. In respect to the former, we show that miR-124 directly targets nuclear factor-κB (NF-κB) p65, and using genetic approaches, we demonstrate that this interaction accounts for the miR-124-mediated suppression of MYC and BCL2. We also characterized miR-124 promoter region and identified a functional p53 binding site. In agreement with this finding, endogenous or ectopic expression of wild-type, but not mutant, p53 increased miR-124 levels and suppressed p65, MYC and BCL2. Our data unveil an miRNA-dependent regulatory circuitry that links p53 to the NF-κB pathway, which when disrupted in B-cell lymphoma may be associated with aberrant coexpression of MYC and BCL2 and poor prognosis.
Summary
In suspension bridges, hanger cables are the main load‐supporting members. The tension of the hanger cables of a suspension bridge is a very important parameter for assessing the integrity and safety of the bridge. In general, indirect methods are used to measure the tension of the hanger cables of a suspension bridge in use. A representative indirect method is the vibration method, which extracts modal frequencies from the cables' responses and then measures the cable tension using the cables' geometric conditions and the modal frequencies. In this study, ambient vibration tests were conducted on a suspension bridge in use to verify the validity of the image‐based back analysis method, which can estimate the tension of remote hanger cables using the modal frequencies as a parameter. The tension estimated through back analysis, which was conducted to minimize the difference between the modal frequencies calculated using finite element analysis of the hanger cables and the measured modal frequencies, was compared with that measured using the vibration method. It was confirmed that reliable tension estimation is possible even with low‐order modal frequencies when the image‐based back analysis method is used.
The occurrence of excessive fluid sloshing during an earthquake can damage structures used to store fluids and can induce secondary disasters, such as environmental destruction and human casualties, due to discharge of the stored fluids. Thus, to prevent such disasters, it is important to accurately predict the sloshing behavior of liquid storage tanks. Tubular level gauges, which visually show the fluid level of a liquid storage tank, are easy to install and economical compared to other water level gauges. They directly show the fluid level and can be applied for various fluids because they can be constructed with various materials according to the fluid characteristics and the intended use. Therefore, in this study, the shaking table test was conducted to verify the validity of the method for measuring the water level response of the tubular level gauge installed on a liquid storage tank using image signals. In addition, image enhancement methods were applied to distinguish between the float installed in the tubular level gauge and the gray level of the background.
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