There is a growing trend to produce lipase from microorganisms owing to their commercial demand in various industries. Bacillus cereus has been shown to have extracellular lipase activity and high growth rates. This study explains the purification of microbial lipase to homogeneity by dialysis, precipitation and chromatography. The purified enzyme with 56kDa relative molecular mass exhibited the highest activity at 60°C (95.56U/ml) and pH 7 (124.50U/ml). The enzyme activity was highly promoted in the presence of K+ (136.17U/ml) and Zn++(133.07 U/ml), and SDS did not affect the enzyme activity, whereas in the company of triton X100 activity of lipase is maximum (23.90 U/ml). The enzyme activity was enhanced by using almond oil (120.00 U/ml) as a substrate. We deduce cheaper protocols for producing extracellular lipase via simple laboratory techniques, which could be a good insight for its production at the commercial level. Keywords: Lipase; Bacillus cereus; chromatography; enzyme activity; purification.
Background: Psoriasis is a skin inflammation characterized by papules and plaques on the skin surface. Almost 125 million people are affected by psoriasis worldwide. This study aimed to evaluate whether the neutrophil-to-lymphocyte ratio (NLR) can be used as a biomarker for the diagnosis of psoriasis. Methods: Blood samples of patients with psoriasis and healthy controls were collected for complete blood count testing. A t-test was applied to estimate the difference in the hematological parameters between the two groups. Results: The hemoglobin level (p < 0.0001), hematocrit level (p < 0.0001), red blood cell count (p < 0.0001), mean corpuscular volume (p = 0.0002), platelet count (p = 0.0039), white blood cell count (p = 0.0002), neutrophil count (p = 0.0001), monocyte count (p < 0.0001), and eosinophil count (p = 0.0467) significantly differed between the groups. Meanwhile, the mean corpuscular hemoglobin concentration (p = 0.3989) and lymphocyte count (p = 0.3842) did not. The patients with psoriasis had a higher neutrophil count and a lower lymphocyte count than the healthy controls. The NLR was significantly elevated in the patients with psoriasis. Conclusion: The NLR is a potential biomarker for the diagnosis of psoriasis.
Background: Hepatitis B virus (HBV) is a major global health concern that can cause both acute and chronic liver infection in humans and cause hepatitis B. The main purpose of our investigation was to show the changes in peripheral hematological parameters as a diagnostic biomarker in hepatitis B patients due to HBV compared to healthy controls. Methods: Blood samples from HBV patients already diagnosed by clinicians and healthy subjects were collected from various hospitals in Punjab. The complete blood count (CBC) test and t test were applied to these samples. Result: The hemoglobin (HB), hematopoietic cell transplantation (HCT), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and neutrophil/lymphocyte ratio (NLR) of patients and controls showed great differences. The white blood cells (WBCs), red blood cells (RBCs), platelets, neutrophils, lymphocytes, monocytes, and eosinophils in hepatitis B patients showed no association. Conclusion: Our primary results indicate a promising biomarker to monitor HBV infection by using information from hematological parameters. Further large cohort investigations are required for more accurate results.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.