Background/aim: Tubal pregnancy is a major cause of maternal death in the first trimester and exploration of its underlying molecular mechanism is of great importance. This study aimed to explore the association of tubal pregnancy with leukemia inhibitory factor (LIF) and leukemia inhibitory factor receptor (LIFR) expression in oviduct tissues.Materials and methods: Immunohistochemistry was performed to probe the differential expression of LIF and LIFR in oviduct tissues among a control group (including NP group, n = 11; and IP group, n = 12), tubal pregnancy group (Ect-N group, n = 31; and Ect-A group, n = 40), and chronically inflamed group (including Inf-S group, n = 11; and Inf-P group, n = 9), followed by semiquantitative analysis.Results: Semiquantitative immunohistochemical analysis demonstrated that there was no significant difference in LIF expression in either the epithelial or stromal cells of oviduct tissues between the tubal pregnancy group and the control group (P < 0.05). However, LIF expression was remarkably elevated in the Inf-S and Inf-P group compared to the other groups (P < 0.05). In the epithelial cells of the fallopian tubes, LIFR expression was highest in the chronically inflamed group, followed by the tubal pregnancy group, outnumbering the control group (P < 0.05). More interestingly, an opposite expression trend of LIFR was observed in the stromal cells of the fallopian tubes among these groups (P < 0.05). Conclusion:Aberrant expression of LIF and LIFR might be associated with the occurrence of tubal pregnancy.
Background: Gestational diabetes mellitus(GDM) is a common obstetric pregnancy complication, which poses a serious threat to the health of pregnant women and newborns. The specific etiology and pathogenesis of this disease have not been fully clarified, it is reported to be related with insulin resistance, inflammatory response and genetic factors etc. Circular RNA(circRNA) is a special kind of non-coding RNA, which have been attracted much attention in recent years. It has been reported that circRNAs may play a regulatory role in pregnancy-related diseases, including GDM. Methods: Previously we reported a circRNA, hsa_circ_005243, which was identified by RNA-sequencing. In this study we detected its expression in 20 GDM pregnant women and 20 normal controls using quantitative reverse transcription polymerase chain reaction analysis. Further in vitro experiments were conducted after hsa_circ_005243 knockdown in HTR8-S/Vneo cells, cell proliferation and migration ability was tested, the secretion of inflammatory factors (TNF-α and IL-6) were detected by ELISA. Then we detected the expression of β-catenin and increased nuclear factor kappa-B (NF-κB) signaling pathways which was related to GDM in the mechanism study. Results: We found the expression of hsa_circ_005243 was significantly reduced both in the placenta and plasma of GDM pregnant women. Knockdown of hsa_circ_005243 in trophoblast cells significantly suppressed cell proliferation and migration ability. In addition, increased secretion of inflammatory factors (TNF-α and IL-6) were observed after hsa_circ_005243 depletion. Further mechanism experiments showed that knockdown of hsa_circ_005243 reduced the expression of β-catenin and increased nuclear NF-κB p65 nuclear translocation. Conclusions: Collectively, our study showed that down-regulation of hsa_circ_005243 might be associated with the pathogenesis of GDM through regulating β-catenin and NF-κB signal pathways and suggest a new potential therapeutic target for GDM.
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