Tuberculosis (TB) is the leading cause of death from a single infectious agent in Korea. In this study, we compared the proteins present in culture filtrates from Mycobacterium tuberculosis strain K, which is the dominant clinical isolate in Korea, with those present in culture filtrates from M. tuberculosis H37Rv. Several differences in expression were detected between the two strains for those proteins with a molecular mass of <20 kDa. ESAT-6, HSP-X, and CFP-10 were found to be abundantly expressed in the strain K culture filtrates by liquid chromatography-electrospray ionization-time of flight mass spectrometry. The serodiagnostic potentials of recombinant antigens rESAT-6, rHSP-X, and rCFP-10 and two native antigens (Ag85 and PstS1) were evaluated by Western blot analysis and enzyme-linked immunosorbent assay (ELISA) using sera collected from 46 TB patients with active disease and 46 healthy controls. As for our ELISA results, HSP-X was superior to the other antigens in terms of sensitivity when a single antigen was employed. The results of a receiver operator characteristic analysis revealed that a cocktail ELISA using all five antigens was significantly more sensitive (77.8%) than the use of a single antigen and offered equivalent specificity; moreover, it produced the largest area under the curve (0.91 versus 0.55 to 0.87). Therefore, a cocktail ELISA containing abundantly expressed antigens enhances the sensitivity of a single antigen and can be a useful diagnostic tool for the detection of active TB.Tuberculosis (TB) is a chronic infectious disease caused by an intracellular pathogen, Mycobacterium tuberculosis, and is a major worldwide health problem. According to the World Health Organization (WHO), one-third of the world's population is latently infected with the bacterium and more than 10 million new cases of TB and 2 million deaths occur across the globe annually (11). Controlling the incidence of TB depends on rapid, sensitive diagnostic tests, efficient drug treatment, and the development of an improved vaccine. Currently, attenuated Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only available vaccine against TB, but it has proven to be problematic because of inconsistent protection and false-positive results during diagnosis due to cross-reactivity (4, 12). The purified protein derivative of M. tuberculosis is the most commonly used antigen for the diagnosis of TB, but its use is also problematic because of poor specificity due to cross-reactivity with antigens from other Mycobacterium species (36). Thus, an individual's BCG vaccination status and the prevalence of environmental mycobacteria are important factors to consider in the diagnosis of TB.Since accurate and reliable diagnostic methods for M. tuberculosis infection are urgently required for the global control of this pathogen, the diagnostic potential of M. tuberculosis-specific antigens has been explored. In an attempt to identify sensitive and specific serodiagnostic antigens or novel immunogenic antigens of M. tuberculosis...
These findings suggest that CysA2 can be used in combination with HspX and/or PstS1 to increase the accuracy of tuberculosis diagnoses.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.