Plants, a rich source of therapeutic compounds because its tremendous applications in the pharmaceutical industry. To find a new and effective antimicrobial compound from the selected plant, the solvents like methanol, Ethanol, Chloroform, Hexane, Xylene, and water were used to evaluate systematically. In vitro antimicrobial activity was performed using Multi-Drug Resistant (MDR) clinical isolates by Kirby Bauer Method. Solvent extracts showed more antimicrobial activity compared to aqueous extracts of both plants. Phytochemical analysis of Piper betel leaves showed the presence of Glycoside, Tannin, Terpenoid, Coumarin, Flavonoid, and Phenol whereas Achyranthes aspera roots were lacking Phenol and Tannin. Thus, present studies suggest the medicinal use of bioactive components from traditional plants Piper betel and Achyranthes aspera in the treatment of various infectious diseases.
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