When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.
HighlightsGiant insulinomas are extremely rare, especially among young patients.Tumor resection is the treatment of choice for giant insulinomas with benign features.Long-term follow-up is essential in benign giant insulinoma to detect the recurrence in the future
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