There is little information available on the in vitro availability of iron jiom commonly consumed green leafy vegetables (GLV) in India. In the present investigation in vitro availability of iron jiom six commonly consumed GLV was calculated @om ionisable ions. Since ascorbic acid and oxalic acid injluence the availability of iron, these constituents were determined and their relationship to the in-vitro availability of the iron content was studied. The effect of uddition of different GLV on the in-vitro availability of iron from a habitual cereal meal was also studied since it is not known how the intrinsic iron of a cereal meal is affected by the addition of these GLV.The GLV studied were amaranth (Amaranthus spinosus), colocasia (Colocasia antiquorum), drumstick (Moringa oleifera), fenugreek (Trigonella foenum-graecum), shepu (Peucedanum graueolens), and spinach (Spinach oleracea). Each of the GLV (80 g) was cleaned, washed and cooked in a minimum amount of distilled water and homogenised, and a suitable aliquot was taken for analysis. The most commonly consumed cereal meal selected for analysis, based upon dietary surveys carried out in Baroda, was wheat chapati and potato. The preparation of the meal, as observed in the homes where the dietary surveys were carried out, was simulated in the laboratory, standard weighed quantities were homogenised and suitable aliquots were taken for analysis.Total iron was estimated using thiocyanate (Wong 1928), and ionisable iron using the in-vitro method proposed by Narasinga Rao and Prabhavathy (1978), and the in-vitro availability of iron was calculated from the ionisable iron using the prediction equation y = 0.4827 + 0.4707x, where y is % in-vitro available iron and x is the % ionisable iron (Narasinga Rao and Prabhavathy 1978). The in-vitro available iron calculated from the in-vitro studies has been shown to correlate well with results from in-vivo studies. Ascorbic acid was estimated by the 2,6-* To whom correspondence should be addressed. 125J Sci Food Agric 0022-5142/88/$03.50
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