Tumor promoters are known to induce reorganization of actin, morphological changes and enhancement of proliferation of epidermal cells in vivo. In this study, we have examined the effects of tumor promoters on these events to clarify the role played by the organization of actin filaments in the regulation of the shape and growth of colonies of epithelial cells in culture. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a change in the shape of colonies of FL and Madin-Darby canine kidney (MDCK) cells within 6 hr. Changes in the shape of colonies were consistent with the morphological change of individual cells and the dissociation of groups of cells in the colonies. Addition of TPA also caused reorganization of actin filaments after 2 hr, and it caused enhancement of proliferation of FL and MDCK cells after 48 hr but did not cause any such changes in KB cells. However, the binding affinities of 4 beta-phorbol 12,13-dibutyrate (PDBu) to FL and MDCK cells were similar to that of PDBu to KB cells. Related tumor promoters such as phorbol 12,13 didecanoate (PDD) and mezerein caused effects similar to those caused by TPA. In contrast, nontumor promoting phorbol esters, such as 4 alpha-PDD and phorbol, had no effect. Cyclic AMP blocked the TPA-induced changes in FL and MDCK cells. These results suggest that TPA-induced reorganization of actin filaments which can be inhibited by cyclic AMP results in changes in the shape of colonies and enhancement of proliferation.

ABSTRACT. We have investigated the effects of various types of collagen and a tumor-promoting phorbol ester on intercellular contacts and the organization of actin in human amnion epithelial FL cells and mouse fibroblast 3T3-A31 cells. Our purpose was to investigate how modulation of interactions between cells and the substratum leads to alterations in intercellular contacts and organization of actin filaments. When cells were cultured on dishes coated with a solution containing type I collagen, but not type IV, changes were induced in the morphology of FL cells and their intercellular contacts. Type I collagen also caused changes in the organization of their actin filaments, although no such effects were observed with 3T3-A31 cells. In contrast, 12-Otetradecanoylphorbol-13-acetate (TPA) caused morphological changes, dissociation of groups of cells, and reorganization of actin filaments in cultures of FL and 3T3-A31 cells. It also disrupted the sites of adhesion of FL cells to the substratum. Both type I collagen and TPA rapidly induced spreading of FL cells in the absence of serum. However, cis-hydroxyproline, known to inhibit secretion of collagen, did not suppress the TPA-induced dissociation of groups of FL cells. These results suggest that the interactions with type I collagen of epithelial FL cells, but not of fibroblastic 3T3-A31 cells, tend to disorganize cellular morphology, intercellular contacts, and actin filaments in ways similar to, but not directly related to, the effects of TPA.Collagen as substratum has been reported to influence the motility (28), proliferation (6, 34), and differentiation (32) of various types of cell in culture through modulation of the interactions between the cells and the substratum. Recently, 12-O-tetradecanoylphorbol-13-acetate (TPA), a potent tumor-promoter, has also been reported to increase the adhesiveness of cells to the substratum (33) and to diminish the number of focal contacts (22). In addition, treatment with TPA modulates the motility, intercellular contacts (7, 31), proliferation, and differentiation of

Impression taking is performed to find model of patient’s teeth and oral cavity in making  restoration or orthodontic appliances. The impression enable bacteria, fungi or virus from saliva or blood in oral cavity to be transferred. Washing the impression by using running water is not enough to completely remove all of bacteria, fungi or virus has been attached on the impression. Therefore the disinfection procedure on impression is needed to avoid the cross infection between the patient and operator. Cross infection of contamination of virus, fungi or bacteria cause of B hepatitis, tuberculosis, herpes zoster and Human Immunodeficiency Virus-Acquired Immune Deficiency Syndrome (HIV-AIDS) diseases. There are many many  disinfectant agents which have been recommended to be used  in effort to disinfect impression such as chlorine compound, iodophore, glutaraldehide and phenol. There are several methods of disinfection can be performed such as immersion, spray or admix the disinfectant into impression material. However several disinfection methods cause side effects such as dimensional change especially on alginate impression, quality of impression surface, surface roughness and other properties of impression material. In this paper the side effects occur in impression material caused by impression disinfection will be studied and discussed. Farther the disinfection by using UV light, blue light, microwave, autoclave, gargle and the possibility of  traditional flora solution using as disinfectant of impression will be explained.

The effects of antiseptic and gargle solutions mixed with the irreversible hydrocolloid alginate impression material to anti-bacterial activity and several properties had been elucidated. The flow property of the such impression has been not elucidated yet. The aim of the study was to evaluate the flow property of alginate impression mixed with gargle solution. Aroma Fine Plus alginate impression material was used and mixed with aquadest as control and Minosep, Betadine and Total Care gargle solutions. Ratio between alginate and each solutions were performed by following the producer’s guidance as 8.4gr alginate and 20ml solution. The every alginate mixtures were made in triplicate (n=3).  Measurement of flow capability was performed by using 0.5ml of alginate mix laid on glass plate covered with cellophane strip and loaded with 1kg metal for 1 minute. The major and minor diameters were measured by using digital caliper. Data were analyzed  by using one way ANOVA (α= 0.05). Aroma Fine mixed with aquadest showed the flow capability was about 26.18mm. Mixed with Minosep showed about 27.85mm, with Betadine 29.19mm and Total Care 27.11mm respectively. Statistically show a significant different  flow capability between aquadest and Betadine mixed impression (p=0.01). Minosep and Total Care gargle solutions showed insignificant different with aquadest mixed impression (p>0.05). It can be concluded that the utilizing of Minosep and Total care gargle solution as  solutions mixed with alginate impression material have no effect to flow property of the impression, except by using Betadine gargle solution.

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