A polymeric ligand, 2-imidazolidinthione-formaldehyde (poly-IF), bearing nitrogen and sulfur donor group was synthesized by the polycondensation of 2-imidazolidinthione and formaldehyde in alkaline medium. The synthetic ligand was characterized with microanalytical and spectral studies. The thermal degradation is investigated by means of dynamic thermogravimetry (TG/DTG) in helium and air atmosphere at various heating rate. The nonisothermal degradation of poly-IF compound was occurred in an inert and air atmosphere. Degradation activation energies were calculated using model free multiple heating rate methods i.e. Flynn-Wall-Ozawa (FWO), Friedman and Kissinger. The compound poly-IF was also tested against strain (Escherichia coli) and it inhibits the proliferation of bacterial growth.