Two bacterial strains, isolates AC10 T and AC20, which were reported in a previous study on the diversity of acetic acid bacteria in Thailand, were subjected to a taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences showed that the two isolates were located closely to the type strains of Gluconobacter oxydans and Gluconobacter roseus. However, the two isolates formed a separate cluster from the type strains of the two species. The genomic DNA of isolate AC10 T was sequenced. The assembled genomes of the isolate were analysed for average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH). The results showed that the highest ANI and dDDH values between isolate AC10 T and G. oxydans DSM 3503 T were 91.15 and 68.2 %, which are lower than the suggested values for species delineation. The genome-based tree was reconstructed and the phylogenetic lineage based on genome sequences showed that the lineage of isolate AC10 T was distinct from G. oxydans DSM 3503 T and its related species. The two isolates were distinguished from G. oxydans and their relatives by their phenotypic characteristics and MALDI-TOF profiles. Therefore, the two isolates, AC10 T (=BCC 15749 T =TBRC 11329 T =NBRC 103576 T ) and AC20 (=BCC 15759=TBRC 11330=NBRC 103579), can be assigned to an independent species within the genus Gluconobacter, and the name Gluconobacter aidae sp. nov. is proposed for the two isolates.
Two Nguyenibacter vanlangensis strains TN01LGIT and VTH-AC01 isolated by an enrichment approach using nitrogen free LGI medium from Asian rice isolation sources are expected as an additional PGPB within the Acetobacteraceae family. In this study, nitrogen fixing capability of the two N. vanlangensis strains was confirmed through nitrogenase activity’s detection by acetylene reduction analysis...
A novel Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium isolated from a sea bean flower [Canavalia rosea (Sw.) DC.] collected in Surat Thani Province, Thailand, and designated as AH18T was characterized on the basis of polyphasic taxonomy. The phylogenetic analysis of 16S rRNA gene revealed that strain AH18T represented a member of the genus
Neokomagataea
. In the 16S rRNA gene sequence analysis, the strain's closest phylogenetic neighbour was
Neokomagataea thailandica
TBRC 376T. The draft genome size of strain AH18T was 2613495 bp, and its DNA G+C content was 52.0 mol%. The strain showed 90.3 and 76.3% pairwise-determined whole-genome average nucleotide identity and 39.8 and 19.6% digital DNA–DNA hybridization values with
N. thailandica
TBRC 376T and
N. tanensis
TBRC 7768T, respectively. The 16S rRNA gene sequences and phylogenomic analysis revealed that the strain clustered with the members of the genus
Neokomagataea
but was located in a distinct branch closely related to
N. thailandica
TBRC 376T. The predominant cellular fatty acids of the strain were summed feature 8 (C18:1
ω6c and/or C18:1
ω7c), C16:0 and C18:1 2OH (>5%). The major respiratory ubiquinone was Q-10. In addition, strain AH18T was substantiated by differences in several physiological characteristics and by MALDI-TOF profiling. On the basis of the results obtained from phenotypic, chemotaxonomic, phylogenetic and genomic analyses, the strain clearly represented a novel species within the genus
Neokomagataea
, for which the name Neokomagataea anthophila sp. nov. (AH18T=TBRC 2177T=NBRC 115156T) is proposed. An emended description of the genus
Neokomagataea
is also given.
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